Vincent A, Kejzlarovà-Lepesant J, Segalat L, Yanicostas C, Lepesant J A
Centre de Recherche de Biochimie et de Génétique Cellulaires, Centre National de la Recherche Scientifique, France.
Mol Cell Biol. 1988 Oct;8(10):4459-68. doi: 10.1128/mcb.8.10.4459-4468.1988.
Low-stringency hybridization of the Drosophila serendipity (sry) finger-coding sequences revealed copies of homologous DNA sequences in the genomes of members of the family Drosophilidae and higher vertebrates. sry h-1, a new Drosophila finger protein-coding gene isolated on the basis of this homology, encodes a 3.2-kilobase (kb) mRNA accumulating in eggs and abundant in early embryos. The predicted sry h-1 protein product, starting at an internal initiation site of translation, is a 868-amino-acid basic polypeptide containing eight TFIIIA-like fingers encoded by three separate exons. Links separating individual fingers in the sry h-1 protein are variable in length and sequence, in contrast with the invariant H/C link found in most multi-fingered proteins. The similarity of the developmental pattern of transcription of sry h-1 with that of several other Drosophila finger protein genes suggests the existence of a complex set of such genes encoding an information which is, at least partly, maternally provided to the embryo and required for activation of gene transcription in early embryos or maintenance of gene activity during subsequent development.
对果蝇意外基因（sry）的指状编码序列进行低严格度杂交，结果显示在果蝇科成员和高等脊椎动物的基因组中存在同源DNA序列的拷贝。基于这种同源性分离出的一个新的果蝇指状蛋白编码基因sry h-1，编码一种3.2千碱基（kb）的mRNA，该mRNA在卵中积累且在早期胚胎中丰富。预测的sry h-1蛋白产物从翻译的内部起始位点开始，是一种868个氨基酸的碱性多肽，包含由三个独立外显子编码的八个TFIIIA样指状结构。与大多数多指蛋白中发现的不变的H/C连接不同，sry h-1蛋白中分隔各个指状结构的连接在长度和序列上是可变的。sry h-1的转录发育模式与其他几个果蝇指状蛋白基因的相似性表明，存在一组复杂的此类基因，它们编码的信息至少部分是由母体提供给胚胎的，并且是早期胚胎中基因转录激活或后续发育过程中基因活性维持所必需的。
