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鸟嘌呤的2-氨基基团对于抗癌抗生素棘霉素与DNA的特异性结合是绝对必需的。

The 2-amino group of guanine is absolutely required for specific binding of the anti-cancer antibiotic echinomycin to DNA.

作者信息

Marchand C, Bailly C, McLean M J, Moroney S E, Waring M J

机构信息

Department of Pharmacology, University of Cambridge, UK.

出版信息

Nucleic Acids Res. 1992 Nov 11;20(21):5601-6. doi: 10.1093/nar/20.21.5601.

DOI:10.1093/nar/20.21.5601
PMID:1454525
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334392/
Abstract

The 2-amino group of guanine is believed to be a critical determinant of potential DNA binding sites for echinomycin and related quinoxaline antibiotics. In order to probe its importance directly we have studied the interaction between echinomycin and DNA species in which guanine N(2) is deleted by virtue of substitution of inosine for guanosine residues. The polymerase chain reaction was used to prepare inosine-substituted DNA. Binding of echinomycin, assessed by DNAse I footprinting, was practically abolished by incorporation of inosine into one or both strands of DNA. We conclude that both the purines in the preferred CpG binding site need to bear a 2-amino group to interact with echinomycin.

摘要

鸟嘌呤的2-氨基被认为是棘霉素及相关喹喔啉类抗生素潜在DNA结合位点的关键决定因素。为了直接探究其重要性，我们研究了棘霉素与DNA物种之间的相互作用，其中鸟嘌呤N(2)因肌苷取代鸟苷残基而缺失。聚合酶链反应用于制备肌苷取代的DNA。通过DNA酶I足迹法评估，棘霉素与DNA的结合在肌苷掺入DNA的一条或两条链时几乎完全消除。我们得出结论，在优选的CpG结合位点中的两个嘌呤都需要带有2-氨基才能与棘霉素相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db3/334392/36fb54e808f1/nar00232-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db3/334392/cd8cd50ea962/nar00232-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db3/334392/eca63dd209cc/nar00232-0093-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db3/334392/36fb54e808f1/nar00232-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db3/334392/cd8cd50ea962/nar00232-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db3/334392/eca63dd209cc/nar00232-0093-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5db3/334392/36fb54e808f1/nar00232-0095-a.jpg

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本文引用的文献

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Reactivity of mitomycin C with synthetic polyribonucleotides containing guanine or guanine analogs.丝裂霉素C与含鸟嘌呤或鸟嘌呤类似物的合成多聚核糖核苷酸的反应活性。
Biochim Biophys Acta. 1982 May 31;697(2):252-4. doi: 10.1016/0167-4781(82)90084-7.
2
Directed biosynthesis of novel derivatives of echinomycin by Streptomyces echinatus. I. Effect of exogenous analogues of quinoxaline-2-carboxylic acid on the fermentation.棘孢链霉菌对棘霉素新型衍生物的定向生物合成。I. 喹喔啉-2-羧酸外源类似物对发酵的影响。
Can J Microbiol. 1984 Apr;30(4):439-50. doi: 10.1139/m84-065.
3
Sequence-specific binding of echinomycin to DNA: evidence for conformational changes affecting flanking sequences.
喹喔啉类抗生素对DNA的识别：利用碱基修饰的DNA分子研究曲奥菌素A和TANDEM序列特异性结合的决定因素。
Biochem J. 1998 Feb 15;330 ( Pt 1)(Pt 1):81-7. doi: 10.1042/bj3300081.
4
Localized chemical reactivity in DNA associated with the sequence-specific bisintercalation of echinomycin.与放线菌素的序列特异性双插入相关的DNA中的局部化学反应性。
Biochem J. 1994 May 15;300 ( Pt 1)(Pt 1):165-73. doi: 10.1042/bj3000165.
5
High resolution mid-prophase human chromosomes induced by echinomycin and ethidium bromide.放线菌素和溴化乙锭诱导产生的高分辨率人类染色体前中期图像
Hum Genet. 1995 Jan;95(1):49-55. doi: 10.1007/BF00225074.
6
The influence of the 2-amino group of guanine on DNA conformation. Uranyl and DNase I probing of inosine/diaminopurine substituted DNA.鸟嘌呤的2-氨基对DNA构象的影响。肌苷/二氨基嘌呤取代DNA的铀酰和DNase I探测。
EMBO J. 1995 May 1;14(9):2121-31. doi: 10.1002/j.1460-2075.1995.tb07204.x.
7
Transferring the purine 2-amino group from guanines to adenines in DNA changes the sequence-specific binding of antibiotics.将DNA中鸟嘌呤的嘌呤2-氨基转移至腺嘌呤会改变抗生素的序列特异性结合。
Nucleic Acids Res. 1995 Mar 25;23(6):885-92. doi: 10.1093/nar/23.6.885.
放线菌素与DNA的序列特异性结合：影响侧翼序列构象变化的证据。
Nucleic Acids Res. 1984 Jun 25;12(12):4865-79. doi: 10.1093/nar/12.12.4865.
4
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Annu Rev Biochem. 1981;50:159-92. doi: 10.1146/annurev.bi.50.070181.001111.
5
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6
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9
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Biochim Biophys Acta. 1987 Jul 14;909(2):145-55. doi: 10.1016/0167-4781(87)90036-4.