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RNA聚合酶III对腺病毒E2E启动子的特异性转录需要TFIID的一个亚群。

Specific transcription from the adenovirus E2E promoter by RNA polymerase III requires a subpopulation of TFIID.

作者信息

Pruzan R, Chatterjee P K, Flint S J

机构信息

Department of Molecular Biology, Princeton University, NJ 08544.

出版信息

Nucleic Acids Res. 1992 Nov 11;20(21):5705-12. doi: 10.1093/nar/20.21.5705.

DOI:10.1093/nar/20.21.5705
PMID:1454534
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334406/
Abstract

The early E2 (E2E) promoter of adenovirus type 2 possesses a TATA-like element and binding sites for the factors E2F and ATF. This promoter is transcribed by RNA polymerase II in high salt nuclear extracts, but by RNA polymerase III in standard nuclear extracts, as judged by sensitivity to low and high, respectively, concentrations of alpha-amanitin. Transcription by the two RNA polymerases initiated at the same site and depended, in both cases, on the TATA-like sequence and upstream elements. However, RNA polymerase III transcripts, unlike those synthesized by RNA polymerase II, terminated at two runs of Ts downstream of the initiation site. Although they are not essential, sequences downstream of the initiation site increased the efficiency of E2E transcription by RNA polymerase III. Such RNA polymerase III dependent transcription required a subpopulation of the general transcription factor, TFIID: TFIID that binds weakly to phosphocellulose (0.3 M eluate) complemented a TFIID-depleted extract to restore RNAp III transcription, whereas TFIID tightly associated with phosphocellulose (1 M eluate) was unable to do so.

摘要

2型腺病毒的早期E2（E2E）启动子具有一个类TATA元件以及E2F和ATF因子的结合位点。根据对低浓度和高浓度α-鹅膏蕈碱的敏感性判断，该启动子在高盐核提取物中由RNA聚合酶II转录，但在标准核提取物中由RNA聚合酶III转录。两种RNA聚合酶的转录均起始于同一位点，且在两种情况下均依赖于类TATA序列和上游元件。然而，与RNA聚合酶II合成的转录本不同，RNA聚合酶III的转录本在起始位点下游的两个连续的T处终止。尽管起始位点下游的序列并非必需，但它们提高了RNA聚合酶III对E2E转录的效率。这种依赖RNA聚合酶III的转录需要一般转录因子TFIID的一个亚群：与磷酸纤维素弱结合（0.3 M洗脱液）的TFIID可补充耗尽TFIID的提取物以恢复RNA聚合酶III的转录，而与磷酸纤维素紧密结合（1 M洗脱液）的TFIID则无法做到这一点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/ac2d32943152/nar00232-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/455cdc86934f/nar00232-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/3c5150a2c88e/nar00232-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/03b70e76d5a6/nar00232-0192-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/595629cf9c35/nar00232-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/cdf215d1f740/nar00232-0193-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/ac2d32943152/nar00232-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/455cdc86934f/nar00232-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/3c5150a2c88e/nar00232-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/03b70e76d5a6/nar00232-0192-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/595629cf9c35/nar00232-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/cdf215d1f740/nar00232-0193-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/372d/334406/ac2d32943152/nar00232-0195-a.jpg

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