Cook J R, Jung V, Schwartz B, Wang P, Pestka S
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854.
Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11317-21. doi: 10.1073/pnas.89.23.11317.
Mutations of the human interferon gamma (IFN-gamma) receptor intracellular domain have permitted us to define a restricted region of that domain as necessary for both induction of class I major histocompatibility complex antigen by IFN-gamma and protection against encephalomyocarditis virus. This region consists of five amino acids (YDKPH), all of which are conserved in the human and murine receptors. Tyr-457 and His-461 are essential for activity. Approximately 80% of the amino acids of the intracellular domain of the receptor is not required for major histocompatibility complex class I antigen induction or for antiviral protection against encephalomyocarditis virus. The observation that there was no protection by IFN-gamma against vesiculostomatitis virus indicates that other factors, in addition to chromosome 21 accessory factor(s), are required to generate the full complement of transduction signals from the human IFN-gamma receptor.
人类干扰素γ（IFN-γ）受体胞内结构域的突变使我们能够确定该结构域中的一个特定区域，该区域对于IFN-γ诱导I类主要组织相容性复合体抗原以及抵御脑心肌炎病毒都是必需的。该区域由五个氨基酸（YDKPH）组成，它们在人类和小鼠受体中均保守。酪氨酸457和组氨酸461对于活性至关重要。受体胞内结构域中约80%的氨基酸对于I类主要组织相容性复合体抗原诱导或抵御脑心肌炎病毒的抗病毒保护并非必需。IFN-γ对水疱性口炎病毒没有保护作用这一观察结果表明，除了21号染色体辅助因子外，还需要其他因子来产生来自人类IFN-γ受体的完整转导信号。
