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使用DNA结合卟啉敏化剂通过658纳米光使λ噬菌体失活。

Inactivation of lambda phage with 658 nm light using a DNA binding porphyrin sensitizer.

作者信息

Kasturi C, Platz M S

机构信息

Department of Chemistry, Ohio State University, Columbus 43210.

出版信息

Photochem Photobiol. 1992 Oct;56(4):427-9. doi: 10.1111/j.1751-1097.1992.tb02184.x.

DOI:10.1111/j.1751-1097.1992.tb02184.x
PMID:1454872
Abstract

Exposure of lambda phage to 658 nm light in the presence of 5,10,15,20-tetrakis-(1-methyl-4-pyridyl)-21H,23H-porphine, tetra-p-tosylate leads to complete (greater than 7 logs) inactivation as measured by the plaque assay. The sensitizer without light and 658 nm photolysis of lambda phage in the absence of sensitizer do not lead to a measurable decrease in viral inactivity. Viral inactivation is not dependent upon the presence of oxygen.

摘要

在5,10,15,20-四(1-甲基-4-吡啶基)-21H,23H-卟啉四对甲苯磺酸盐存在的情况下,将λ噬菌体暴露于658 nm光下会导致完全(大于7个对数)失活,这通过噬菌斑测定法来衡量。没有光时的敏化剂以及在没有敏化剂的情况下对λ噬菌体进行658 nm光解,均不会导致病毒活性出现可测量的下降。病毒失活不依赖于氧气的存在。

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