Suntornsuk Leena, Pipitharome Ongart, Wilairat Prapin
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhaya Rd., Rajathevee, Bangkok 10400, Thailand.
J Pharm Biomed Anal. 2003 Oct 15;33(3):441-9. doi: 10.1016/s0731-7085(03)00288-7.
A micellar electrokinetic chromatography (MEKC) method was established for determination of paracetamol (PARA) and chlorpheniramine maleate (CPM) in cold tablets. Separation of both drugs, as well as other seven cold remedy ingredients, was achieved in 25.5 min using a sodium dihydrogenphosphate-sodium tetraborate buffer (10 mM, pH 9.0) containing sodium dodecyl sulfate (SDS) (50 mM) and acetonitrile (26% v/v). The effective capillary length of 50 cm, the separating voltage of 15 kV and the temperature of 30 degrees C was optimized. Detection was by a diode array detector at 214 nm. Method linearity was excellent (r(2)>0.999) over the concentration tested (10-250 microg/ml) with good precision and accuracy. Recoveries were good (>99%) with limits of detection of 0.4 and 0.5 microg/ml and limits of quantitation of 2 (%R.S.D.=3.1%) and 4 (%R.S.D.=2.4%) microg/ml, for PARA and CPM, respectively. The developed method was applied to the determination of ingredients in cold tablets and was found to be simple, rapid and efficient.
建立了胶束电动色谱法（MEKC）用于测定感冒片中的对乙酰氨基酚（PARA）和马来酸氯苯那敏（CPM）。使用含有十二烷基硫酸钠（SDS）（50 mM）和乙腈（26% v/v）的磷酸二氢钠-硼砂缓冲液（10 mM，pH 9.0），在25.5分钟内实现了两种药物以及其他七种感冒治疗成分的分离。优化了有效毛细管长度为50 cm、分离电压为15 kV和温度为30℃的条件。采用二极管阵列检测器在214 nm波长处进行检测。在所测试的浓度范围（10 - 250 μg/ml）内，方法线性良好（r²>0.999），精密度和准确度高。对乙酰氨基酚和马来酸氯苯那敏的回收率良好（>99%），检测限分别为0.4和0.5 μg/ml，定量限分别为2（相对标准偏差=3.1%）和4（相对标准偏差=2.4%）μg/ml。所建立的方法应用于感冒片中成分的测定，结果表明该方法简便、快速且高效。
