芽孢杆菌的类核酮糖-1,5-二磷酸羧化酶/加氧酶蛋白与光合核酮糖-1,5-二磷酸羧化酶/加氧酶之间的功能联系。

A functional link between RuBisCO-like protein of Bacillus and photosynthetic RuBisCO.

作者信息

Ashida Hiroki, Saito Yohtaro, Kojima Chojiro, Kobayashi Kazuo, Ogasawara Naotake, Yokota Akiho

机构信息

Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama, Ikoma, Nara 630-0101, Japan.

出版信息

Science. 2003 Oct 10;302(5643):286-90. doi: 10.1126/science.1086997.

DOI:10.1126/science.1086997

PMID:14551435

Abstract

The genomes of several nonphotosynthetic bacteria, such as Bacillus subtilis, and some Archaea include genes for proteins with sequence homology to the large subunit of ribulose bisphosphate carboxylase/oxygenase (RuBisCO). We found that such a RuBisCO-like protein (RLP) from B. subtilis catalyzed the 2,3-diketo-5-methylthiopentyl-1-phosphate enolase reaction in the methionine salvage pathway. A growth-defective mutant, in which the gene for this RLP had been disrupted, was rescued by the gene for RuBisCOfrom the photosynthetic bacterium Rhodospirillum rubrum. Thus, the photosynthetic RuBisCO from R. rubrum retains the ability to function in the methionine salvage pathway in B. subtilis.

摘要

几种非光合细菌（如枯草芽孢杆菌）以及一些古生菌的基因组中包含与二磷酸核酮糖羧化酶/加氧酶（RuBisCO）大亚基具有序列同源性的蛋白质基因。我们发现，枯草芽孢杆菌中的这种类RuBisCO蛋白（RLP）在甲硫氨酸补救途径中催化2,3-二酮-5-甲基硫代戊基-1-磷酸烯醇酶反应。一个生长缺陷型突变体，其RLP基因已被破坏，通过来自光合细菌红螺菌的RuBisCO基因得以挽救。因此，红螺菌的光合RuBisCO保留了在枯草芽孢杆菌甲硫氨酸补救途径中发挥作用的能力。