Bettini G, Morini M, Marcato P S
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Servizio di Anatomia Patologica, Facoltà di Medicina Veterinaria, Alma Mater Studiorum Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia Bologna, Italy.
J Comp Pathol. 2003 Nov;129(4):283-93. doi: 10.1016/s0021-9975(03)00046-x.
To assess the relevance of spindle cell tumours in the canine gastrointestinal (GI) tract and to classify them, a retrospective study was carried out on haematoxylin and eosin-stained sections from formalin-fixed paraffin wax-embedded samples of 105 primary GI tumours. Seventeen out of 105 (16%) GI tumours were mesenchymal, 48% were epithelial and 36% were round cell tumours. Spindle cell tumours were stained by Masson trichrome, Orcein-Van Gieson and labelled immunohistochemically (vimentin, desmin, smooth muscle actin, protein S100, glial fibrillar acid protein, CD117 and MIB-1) and the histological grade, mitotic index, nuclear size and cellular density were also assessed. The 17 gastrointestinal mesenchymal tumours were classified as 10 leiomyomas (10/10 positive for desmin and smooth muscle actin; 6/10 positive for vimentin) 2 leiomyosarcomas (2/2 positive for desmin, smooth muscle actin and vimentin) and 5 gastrointestinal stromal tumours (GISTs) (5/5 positive for CD117 and vimentin; 3/5 positive for smooth muscle actin). Canine GISTs appeared as densely packed spindle cell tumours, with a diffuse, strong, cytoplasmic immunopositivity for c-kit protein (CD117). GISTs, defined as CD117-positive spindle cell or epithelioid or pleomorphic neoplasms that presumably derive from interstitial cells of Cajal, are reported in recent medical studies as the most common mesenchymal tumours of the GI tract. Our data suggest that GISTs represent a significant portion of canine GI spindle cell tumours, which can be definitely distinguished from leiomyosarcomas only by their expression of CD117.
为评估犬胃肠道（GI）纺锤状细胞肿瘤的相关性并对其进行分类，我们对105例原发性GI肿瘤的福尔马林固定石蜡包埋样本的苏木精和伊红染色切片进行了回顾性研究。105例GI肿瘤中，17例（16%）为间充质肿瘤，48%为上皮性肿瘤，36%为圆形细胞肿瘤。对纺锤状细胞肿瘤进行了Masson三色染色、orcein - Van Gieson染色及免疫组织化学标记（波形蛋白、结蛋白、平滑肌肌动蛋白、蛋白S100、胶质纤维酸性蛋白、CD117和MIB - 1），并评估了组织学分级、有丝分裂指数、核大小和细胞密度。17例胃肠道间充质肿瘤分类如下：10例平滑肌瘤（10/10结蛋白和平滑肌肌动蛋白阳性；6/10波形蛋白阳性），2例平滑肌肉瘤（2/2结蛋白、平滑肌肌动蛋白和波形蛋白阳性），5例胃肠道间质瘤（GISTs）（5/5 CD117和波形蛋白阳性；3/5平滑肌肌动蛋白阳性）。犬GISTs表现为紧密排列的纺锤状细胞肿瘤，对c - kit蛋白（CD117）呈弥漫性、强细胞质免疫阳性。GISTs被定义为CD117阳性的纺锤状细胞、上皮样或多形性肿瘤，据推测起源于Cajal间质细胞，在最近的医学研究中被报道为胃肠道最常见的间充质肿瘤。我们的数据表明，GISTs占犬GI纺锤状细胞肿瘤的很大一部分，仅通过其CD117表达就能与平滑肌肉瘤明确区分。
