LaRock R G, Ginn P E
Department of Pathobiology, University of Florida, College of Veterinary Medicine, Gainesville 32611-0880, USA.
Vet Pathol. 1997 Jul;34(4):303-11. doi: 10.1177/030098589703400406.
Sections from 35 formalin-fixed, paraffin-embedded, canine gastrointestinal stromal tumors consisting of 14 leiomyomas (five stomach, three small intestine, two colon, four rectum), 18 leiomyosarcomas (one stomach, five small intestine, nine cecum, three rectum), two undifferentiated sarcomas (two stomach), and one neurofibrosarcoma (small intestine) were examined for the expression of vimentin, S-100 protein, alpha-smooth muscle actin, and desmin via immunoperoxidase methodology using an avidin-biotin complex technique. The leiomyomas were 4/14 (29%) vimentin-positive, 3/14 (21%) S-100 protein-positive, 10/14 (71%) alpha-smooth muscle actin-positive and 13/14 (93%) desmin-positive. Leiomyosarcomas were 18/18 (100%) vimentin-positive, 11/18 (61%) S-100 protein-positive, 9/18 (50%) a-smooth muscle actin-positive, and 15/18 (83%) desmin-positive. The undifferentiated sarcomas were 2/2 (100%) vimentin-positive, 2/2 (100%) S-100 protein-positive, 1/2 (50%) alpha-smooth muscle actin-positive, and 0/2 (0%) desmin-positive. The neurofibrosarcoma was vimentin and S-100 protein-positive and alpha-smooth muscle actin- and desmin-negative. Thirty-one of thirty-five (89%) of all neoplasms demonstrated reactivity for either desmin and/or alpha-smooth muscle actin. S-100 protein reactivity occurred in 17/35 (49%) of all specimens. Lack of desmin and alpha-smooth muscle actin reactivity occurred in 4/35 (11%) of all specimens, all of which were vimentin-positive. The immunohistochemical results indicate that the majority of canine gastrointestinal stromal tumors (GIST) with light microscopic features of smooth muscle cells have immunohistochemical staining patterns supporting smooth muscle differentiation. Vimentin reactivity correlated with a light microscopic diagnosis of malignancy. The lack of smooth muscle cell markers in some tumors and the high percentage of cases positive for S-100 protein may suggest a more complex histogenesis or differentiation for subgroups of these tumors.
选取35例福尔马林固定、石蜡包埋的犬胃肠道间质瘤组织切片进行研究，其中包括14例平滑肌瘤（5例位于胃，3例位于小肠，2例位于结肠，4例位于直肠）、18例平滑肌肉瘤（1例位于胃，5例位于小肠，9例位于盲肠，3例位于直肠）、2例未分化肉瘤（均位于胃）以及1例神经纤维肉瘤（位于小肠）。采用抗生物素蛋白-生物素复合物技术，通过免疫过氧化物酶法检测波形蛋白、S-100蛋白、α-平滑肌肌动蛋白和结蛋白的表达情况。平滑肌瘤中，波形蛋白阳性率为4/14（29%），S-100蛋白阳性率为3/14（21%），α-平滑肌肌动蛋白阳性率为10/14（71%），结蛋白阳性率为13/14（93%）。平滑肌肉瘤中，波形蛋白阳性率为18/18（100%），S-100蛋白阳性率为11/18（61%），α-平滑肌肌动蛋白阳性率为9/18（50%），结蛋白阳性率为15/18（83%）。未分化肉瘤中，波形蛋白阳性率为2/2（100%），S-100蛋白阳性率为2/2（100%），α-平滑肌肌动蛋白阳性率为1/2（50%），结蛋白阳性率为0/2（0%）。神经纤维肉瘤波形蛋白和S-100蛋白阳性，α-平滑肌肌动蛋白和结蛋白阴性。所有肿瘤中，35例中有31例（89%）对结蛋白和/或α-平滑肌肌动蛋白呈反应性。17/35（49%）的所有标本出现S-100蛋白反应性。所有标本中，4/35（11%）缺乏结蛋白和α-平滑肌肌动蛋白反应性，这些均为波形蛋白阳性。免疫组织化学结果表明，大多数具有平滑肌细胞光镜特征的犬胃肠道间质瘤（GIST）具有支持平滑肌分化的免疫组织化学染色模式。波形蛋白反应性与光镜下的恶性诊断相关。一些肿瘤中缺乏平滑肌细胞标志物以及S-100蛋白阳性病例的高比例可能提示这些肿瘤亚组的组织发生或分化更为复杂。
