Priault Muriel, Camougrand Nadine, Kinnally Kathleen W, Vallette François M, Manon Stéphen
IBGC/CNRS, 1 Rue Camille Saint-Saëns, Université de Bordeaux 2, F-33077 Bordeaux, France.
FEMS Yeast Res. 2003 Oct;4(1):15-27. doi: 10.1016/S1567-1356(03)00143-0.
The budding yeast Saccharomyces cerevisiae has proven to be a powerful tool in investigations of the molecular aspects of the events involved in apoptosis, particularly the steps implicating mitochondria. Yeast does not have obvious homologs of the proteins involved in the regulation of apoptosis, and provides a simplified model system in which the function of these proteins can be unraveled. This review focuses on the interactions of two of the major pro-apoptotic Bcl-2 family members, Bax and Bid, with mitochondria. It is shown that yeast has allowed questioning of several crucial aspects of the function of these two proteins, namely the molecular mechanisms driving their insertion into the mitochondrial outer membrane and those leading to the permeabilization to cytochrome c. More recently, signaling pathways leading to Bax-induced cell death, as well as other forms of cell death, have been identified in yeast. Both 'apoptosis-like' and autophagy-related forms of cell degradation are involved, and mitochondria play a central role in these two signaling pathways.
出芽酵母酿酒酵母已被证明是研究细胞凋亡相关事件分子层面，特别是涉及线粒体的步骤的有力工具。酵母没有参与细胞凋亡调控的蛋白质的明显同源物，并提供了一个简化的模型系统，在其中可以阐明这些蛋白质的功能。本综述重点关注两个主要的促凋亡Bcl-2家族成员Bax和Bid与线粒体的相互作用。结果表明，酵母使得人们能够对这两种蛋白质功能的几个关键方面提出质疑，即驱动它们插入线粒体外膜的分子机制以及导致细胞色素c通透化的机制。最近，在酵母中已经鉴定出导致Bax诱导的细胞死亡以及其他形式细胞死亡的信号通路。“类凋亡”和自噬相关形式的细胞降解均涉及其中，并且线粒体在这两条信号通路中都起着核心作用。
