Ishii Yoshimasa, Ushida Takashi, Tateishi Tetsuya, Miyanaga Yutaka
3D Tissue Engineering Group, National Institute of Advanced Industrial Science and Technology, Japan.
J Orthop Res. 2003 Nov;21(6):1113-7. doi: 10.1016/S0736-0266(03)00056-1.
The effects of intermittent exposure to oxygen injection on an experimentally induced ligament tear were studied in the right hind limb of 17 male Sprague-Dawley rats. Two rats were used for monitoring the partial oxygen pressure (pO(2)) of subcutaneous tissue and 15 rats were divided into the following three groups of 5 after an experimentally induced ligament tear: Group A, control group; Group B, injection of 0.5 ml hyaluronan to the wound transcutaneously; Group C, injection of 0.5 ml hyaluronan mixed with haemoglobin and oxygen (n=5). At 7 days post-ligament injury, we compared the ligaments of the three treatment groups for gross appearance, histology and expression of vascular endothelial growth factor (VEGF) mRNA by RT-PCR. Our results indicate that the pO(2) was immediately elevated to 334.6 mmHg by topical oxygen injection and this method was effective in promoting vessel formation in comparison to the control group (p<0.01). However, the expression of VEGF mRNA in the topical oxygen injection group (Group C) was lower than that in control group (p<0.05). Our results suggest that oxygen is able to accelerate vessel formation in spite of its effect of decreasing VEGF mRNA. Our method of using topical injection proved to be useful in healing the ligament and the wound.
在17只雄性Sprague-Dawley大鼠的右后肢上，研究了间歇性注氧对实验性诱导的韧带撕裂的影响。两只大鼠用于监测皮下组织的局部氧分压（pO₂），15只大鼠在实验性诱导韧带撕裂后被分为以下三组，每组5只：A组，对照组；B组，经皮向伤口注射0.5 ml透明质酸；C组，注射0.5 ml混合有血红蛋白和氧气的透明质酸（n = 5）。在韧带损伤后7天，我们通过RT-PCR比较了三个治疗组韧带的大体外观、组织学以及血管内皮生长因子（VEGF）mRNA的表达。我们的结果表明，局部注氧可使pO₂立即升高至334.6 mmHg，与对照组相比，该方法在促进血管形成方面有效（p<0.01）。然而，局部注氧组（C组）中VEGF mRNA的表达低于对照组（p<0.05）。我们的结果表明，尽管氧有降低VEGF mRNA的作用，但它仍能够加速血管形成。我们使用局部注射的方法被证明对韧带和伤口的愈合有用。
