Byrd W, Harmon B G, Kadis S
Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Vet Immunol Immunopathol. 1992 Nov;34(3-4):307-24. doi: 10.1016/0165-2427(92)90172-m.
In an attempt to protect pigs against swine pleuropneumonia induced by Actinobacillus pleuropneumoniae (SPAP) by neutralizing the effects of three virulence factors of A. pleuropneumoniae--the capsular polysaccharide (CP), the lipopolysaccharide (LPS), and the hemolysin protein (HP)--two subunit conjugate vaccines were prepared by covalently coupling the CP to the HP and the LPS to the HP. The CP, LPS, and HP were isolated from A. pleuropneumoniae, strain 4074, serotype 1, and the protective efficacy of the conjugate vaccines in swine experimentally infected with A. pleuropneumoniae was evaluated. Following a booster vaccination, a significant (P < 0.05) IgG antibody response to the CP, LPS, and HP was detected in the vaccinated pigs. The pigs vaccinated with the CP-HP and LPS-HP conjugates exhibited significantly less mortality (P < 0.05) and significantly greater weight gain (P < 0.001) than unvaccinated pigs. Vaccinated pigs exhibited significantly fewer and less extensive gross pulmonary lesions (P < 0.001) when compared with unvaccinated pigs. Thus, on the basis of mortality, weight gains, and pulmonary lesion formation, the two conjugate vaccines used in conjunction with one another provide noticeable protective efficacy against SPAP.
为了通过中和胸膜肺炎放线杆菌的三种毒力因子——荚膜多糖（CP）、脂多糖（LPS）和溶血素蛋白（HP）的作用来保护猪免受胸膜肺炎放线杆菌诱导的猪胸膜肺炎（SPAP），通过将CP与HP共价偶联以及将LPS与HP共价偶联制备了两种亚单位结合疫苗。CP、LPS和HP从血清型1的胸膜肺炎放线杆菌4074菌株中分离出来，并评估了结合疫苗对实验感染胸膜肺炎放线杆菌的猪的保护效力。加强免疫后，在接种疫苗的猪中检测到对CP、LPS和HP有显著（P<0.05）的IgG抗体反应。与未接种疫苗的猪相比，接种CP-HP和LPS-HP结合物的猪死亡率显著降低（P<0.05），体重增加显著更大（P<0.001）。与未接种疫苗的猪相比，接种疫苗的猪肺部肉眼可见病变显著更少且范围更小（P<0.001）。因此，基于死亡率、体重增加和肺部病变形成情况，两种结合疫苗联合使用对SPAP具有显著的保护效力。
