[幽门螺杆菌感染与不同胃黏膜病变中c-myc、Bcl-2及Bax蛋白表达的关系]

[Relationship between Helicobacter pylori infection and expression of c-myc, Bcl-2, and Bax protein in different gastric mucosa lesions].

作者信息

Zhan Na, Xiong Yong-Yan, Lan Jing, Wang Bi-Cheng, Tian Su-Fang, Yu Shao-Ping

机构信息

Department of Pathology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei, 430071, PR China.

出版信息

Ai Zheng. 2003 Oct;22(10):1034-7.

PMID:14558945

Abstract

BACKGROUND & OBJECTIVE: Helicobacter pylori (HP) has been believed to be a carcinogen of gastric carcinoma. However, its mechanism was yet not clearly understood. This study was designed to investigate the relationship between HP infection and gastric epithelial cell proliferation as well as apoptosis in different gastric mucosa lesions and elucidate the probable mechanism of gastric carcinogenesis relating with HP infection.

METHODS

A total of 272 cases were available for the study including 42 cases of chronic gastritis (CG), 46 cases of intestinal metaplasia I or II (IM I- II), 25 cases of intestinal metaplasia III (IM III), 21 cases of mild dysplasia (Dys I), 54 cases of modest or severe dysplasia (Dys II- III), and 84 cases of gastric cancer (GC). HP infection was detected by Warthin-Starry bacterium staining method and streptavidin-peroxidase (SP) immunohistochemical method. HID-AB(pH2.5)- PAS method was used to define the quality of mucus. The expression of c-myc, Bcl-2, and Bax were detected using SP immunohistochemical method. The chi-square test and the Fisher's exact probability test were used to compare the frequencies.

RESULTS

(1)The expression of c-myc and Bcl-2 increased as gastric mucosa lesions developed from CG,IM,Dys to GC,but the expression of Bax decreased. The expression of c-myc was significantly higher in GC than that in Dys II- III and IM III(all P< 0.01), but the expression of Bax was significantly lower in GC than that in Dys II- III and IM III(P< 0.05 or P< 0.01). (2)The expression of c-myc in IM III and Dys II- III with HP infection was 62.50% and 66.67%,respectively, significantly higher than that without infection(11.11%,27.78%,all P< 0.05). The expression of Bax in CG, IM I- II and IM III with HP infection were 87.10%, 81.25%, and 62.50%, respectively, significantly higher than those without infection (54.55%, 42.86%, 11.11%, all P< 0.05). Furthermore HP infection was associated with the expression of Bcl-2 in IM III, Dys II- III and GC (P< 0.05 or P< 0.01).

CONCLUSION

HP infection can cause serious imbalance between cell proliferation and apoptosis in the precancerous lesions (IM III and Dys II- III), giving chances for gastric carcinogenesis.

摘要

背景与目的

幽门螺杆菌（HP）一直被认为是胃癌的致癌因子。然而，其机制尚未完全明确。本研究旨在探讨HP感染与不同胃黏膜病变中胃上皮细胞增殖及凋亡的关系，阐明HP感染相关胃癌发生的可能机制。

方法

本研究共纳入272例患者，包括42例慢性胃炎（CG）、46例Ⅰ或Ⅱ级肠化生（IM I-II）、25例Ⅲ级肠化生（IM III）、21例轻度不典型增生（Dys I）、54例中度或重度不典型增生（Dys II-III）以及84例胃癌（GC）。采用Warthin-Starry细菌染色法和链霉抗生物素蛋白-过氧化物酶（SP）免疫组织化学方法检测HP感染情况。采用HID-AB（pH2.5）-PAS方法确定黏液质量。采用SP免疫组织化学方法检测c-myc、Bcl-2和Bax的表达。采用卡方检验和Fisher确切概率法比较频率。

结果

（1）随着胃黏膜病变从CG、IM、Dys发展至GC，c-myc和Bcl-2的表达增加，而Bax的表达降低。GC中c-myc的表达显著高于Dys II-III和IM III（均P<0.01），但GC中Bax的表达显著低于Dys II-III和IM III（P<0.05或P<0.01）。（2）HP感染的IM III和Dys II-III中c-myc的表达分别为62.50%和66.67%，显著高于未感染组（11.11%、27.78%，均P<0.05）。HP感染的CG、IM I-II和IM III中Bax的表达分别为87.10%、81.25%和62.50%，显著高于未感染组（54.55%、42.86%、11.11%，均P<0.05）。此外，HP感染与IM III、Dys II-III和GC中Bcl-2的表达相关（P<0.05或P<0.01）。