Dimov V, Ivanovska N, Bankova V, Popov S
Department of Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
Vaccine. 1992;10(12):817-23. doi: 10.1016/0264-410x(92)90043-j.
The efficacy of the water-soluble derivative (WSD) of natural propolis (bee glue) was examined for augmentation of host resistance against experimental infections caused by Gram-negative pathogens (Klebsiella pneumoniae, Proteus vulgaris, Escherichia coli, Pseudomonas aeruginosa). The substance was found to induce significant non-specific protection, but did not inhibit the in vitro growth of the same strains. Pretreatment with WSD prior to the standard scheme for tumour necrosis factor (TNF) induction (BCG and two weeks later lipopolysaccharide (LPS)) provoked an interval-dependent reduction in the lytic capacity of serum against L 929 target cells. The replacement of the triggering or priming signal with WSD markedly increased TNF production. In vivo administration of WSD led to a rapid and route-dependent change in the alternative complement pathway haemolysis. The alteration in C1q complement component and total protein synthesis, and also in nitroblue tetrazolium reduction, suggests that macrophage activation makes a major contribution to the capacity of WSD to prevent infections.
研究了天然蜂胶（蜂胶）的水溶性衍生物（WSD）增强宿主对革兰氏阴性病原体（肺炎克雷伯菌、普通变形杆菌、大肠杆菌、铜绿假单胞菌）所致实验性感染抵抗力的效果。发现该物质可诱导显著的非特异性保护作用，但不抑制相同菌株的体外生长。在诱导肿瘤坏死因子（TNF）的标准方案（卡介苗，两周后注射脂多糖（LPS））之前用WSD预处理，会导致血清对L 929靶细胞的溶解能力呈时间间隔依赖性降低。用WSD替代触发或启动信号可显著增加TNF的产生。WSD的体内给药导致替代补体途径溶血迅速且依途径发生变化。C1q补体成分、总蛋白合成以及硝基蓝四氮唑还原的改变表明，巨噬细胞激活对WSD预防感染的能力起主要作用。
