Cervin A, Lindberg S, Mercke U, Uddman R
Department of Oto-Rhino-Laryngology, University Hospital, Lund, Sweden.
Acta Otolaryngol. 1992 Sep;112(5):872-81. doi: 10.3109/00016489209137486.
The distribution of neuropeptide Y (NPY)-immunoreactivity was investigated in the rabbit maxillary sinus and adjacent ganglia. A moderate supply of NPY-containing nerve fibers occurred around seromucous glands and a denser supply around small blood vessels. Only a few immunoreactive nerve fibers were seen beneath the epithelium. Double immunostaining showed that vasoactive intestinal peptide (VIP) coexisted with NPY in the nerve fibers surrounding blood vessels and seromucous glands. NPY-containing nerve cell bodies were numerous in the superior cervical ganglion, and moderately numerous in the sphenopalatine ganglion. The finding of NPY-containing neurons in the latter parasympathetic ganglion suggests that NPY may influence the cholinergic regulation of mucociliary activity. The effect of NPY on the mucociliary activity of the maxillary sinus in connection with cholinergic stimulation has therefore been investigated in vivo using a photoelectric technique. At dosages of 2.5 and 5.0 micrograms/kg, the ganglionic stimulant nicotine bitartrate, which increases mucociliary activity by a cholinergic pathway, accelerated mucociliary activity by 28.0 +/- 7.5% and 36.8 +/- 6.2%, respectively. In the same experiment repeated during infusion of NPY (0.1 microgram/kg/min), the increase in mucociliary activity was reduced to 10.8 +/- 2.3% and 28.9 +/- 7.1%, respectively. Infusion of NPY did not affect the stimulating effect on mucociliary activity by bolus injections (0.1 and 0.5 microgram/kg) of the cholinergic agonist, methacholine. It is concluded that NPY-like immunoreactivity is present in nerve fibers in the rabbit maxillary sinus and in neurons in the sympathetic and parasympathetic ganglia that supply the nose and paranasal sinuses. NPY attenuates the effect of nicotine on mucociliary activity, probably via a prejunctional mechanism, and may act as a modulator of cholinergic regulation of the mucociliary system.
研究了兔上颌窦及相邻神经节中神经肽Y（NPY）免疫反应性的分布。在浆液性腺周围有适量含NPY的神经纤维供应，在小血管周围供应更为密集。上皮下方仅可见少数免疫反应性神经纤维。双重免疫染色显示，血管活性肠肽（VIP）与NPY在血管和浆液性腺周围的神经纤维中共存。含NPY的神经细胞体在上颈神经节中大量存在，在蝶腭神经节中数量适中。在后一个副交感神经节中发现含NPY的神经元表明，NPY可能影响纤毛黏液活性的胆碱能调节。因此，利用光电技术在体内研究了NPY对与胆碱能刺激相关的上颌窦纤毛黏液活性的影响。在剂量为2.5和5.0微克/千克时，通过胆碱能途径增加纤毛黏液活性的神经节兴奋剂重酒石酸尼古丁分别使纤毛黏液活性加速了28.0±7.5%和36.8±6.2%。在输注NPY（0.1微克/千克/分钟）期间重复进行的同一实验中，纤毛黏液活性的增加分别降至10.8±2.3%和28.9±7.1%。输注NPY不影响胆碱能激动剂乙酰甲胆碱大剂量注射（0.1和0.5微克/千克）对纤毛黏液活性的刺激作用。得出结论，兔上颌窦神经纤维以及支配鼻和鼻窦的交感和副交感神经节神经元中存在NPY样免疫反应性。NPY可能通过节前机制减弱尼古丁对纤毛黏液活性的影响，并可能作为纤毛黏液系统胆碱能调节的调节剂。
