Grunditz T, Ekman R, Håkanson R, Sundler F, Uddman R
Department of Oto-Rhino-Laryngology, Malmö General Hospital, Sweden.
Regul Pept. 1988 Nov;23(2):193-208. doi: 10.1016/0167-0115(88)90027-4.
Neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP) occur in nerve fibers around blood vessels and between follicles in the thyroid gland of the mouse and rat. VIP-immunoreactive fibers are numerous, while NPY-immunoreactive fibers are fewer. Most of the latter fibers contain noradrenaline (NA) as well as NPY, while a subpopulation was found to contain VIP instead of NA. We have determined the origins of rat thyroid nerve fibers containing NPY, VIP or NPY/VIP by investigating 3 conceivable sources, i.e. the superior cervical ganglion, the nodose ganglion and the thyroid ganglion. Chemical sympathectomy or removal of the superior cervical ganglion did not affect the frequency of VIP-immunoreactive fibers but eliminated most of the NPY-immunoreactive fibers as well as all NA-containing nerve fibers (recognized by antibodies to dopamine-beta-hydroxylase). The NPY-immunoreactive fibers that remained after sympathectomy occurred around blood vessels and between follicles and contained VIP. Cervical vagotomy (removal of the nodose ganglion including the adjacent vagus) did not overtly affect the frequency of NPY/VIP-, VIP-, or NPY/NA-containing fibers in the thyroid. In contrast, extirpation of the thyroid ganglion, which is situated immediately outside the thyroid capsule, greatly reduced the number of VIP- and NPY/VIP-containing fibers in the rat thyroid. On the whole, the results of radioimmunoassay of NPY and VIP agreed well with the immunocytochemical findings. High performance liquid chromatography confirmed the identity of NPY and VIP. The present findings suggest the existence in the rat thyroid of one NPY-containing nerve fiber population that harbours NA and emanates from the superior cervical ganglion; one NPY-containing fiber population that is non-adrenergic, harbours VIP and originates in the thyroid ganglion; and a second VIP-containing fiber population that is devoid of NPY and appears to derive from the thyroid ganglion.
神经肽Y（NPY）和血管活性肠肽（VIP）存在于小鼠和大鼠甲状腺中血管周围以及滤泡之间的神经纤维中。VIP免疫反应性纤维数量众多，而NPY免疫反应性纤维较少。大多数后一种纤维同时含有去甲肾上腺素（NA）和NPY，而发现一小部分纤维含有VIP而非NA。我们通过研究3种可能的来源，即颈上神经节、结状神经节和甲状腺神经节，来确定大鼠甲状腺中含NPY、VIP或NPY/VIP的神经纤维的起源。化学交感神经切除术或切除颈上神经节并不影响VIP免疫反应性纤维的数量，但消除了大多数NPY免疫反应性纤维以及所有含NA的神经纤维（通过多巴胺-β-羟化酶抗体识别）。交感神经切除术后残留的NPY免疫反应性纤维出现在血管周围和滤泡之间，并且含有VIP。颈迷走神经切断术（切除结状神经节包括相邻的迷走神经）并未明显影响甲状腺中含NPY/VIP、VIP或含NPY/NA纤维的数量。相比之下，位于甲状腺囊外紧邻处的甲状腺神经节切除，大大减少了大鼠甲状腺中含VIP和含NPY/VIP纤维的数量。总体而言，NPY和VIP的放射免疫测定结果与免疫细胞化学结果非常吻合。高效液相色谱法证实了NPY和VIP的特性。目前的研究结果表明，大鼠甲状腺中存在一种含NPY的神经纤维群体，其含有NA并起源于颈上神经节；一种含NPY的非肾上腺素能纤维群体，含有VIP并起源于甲状腺神经节；以及第二种不含NPY的含VIP纤维群体，似乎也起源于甲状腺神经节。
