de Cremoux Patricia, Coste Joël, Sastre-Garau Xavier, Thioux Martine, Bouillac Christelle, Labbé Sylvain, Cartier Isabelle, Ziol Marianne, Dosda Anne, Le Galès Catherine, Molinié Vincent, Vacher-Lavenu Marie-Cécile, Cochand-Priollet Béatrix, Vielh Philippe, Magdelénat Henri
Department of Tumor Biology, Institut Curie, Paris, France.
Am J Clin Pathol. 2003 Oct;120(4):492-9. doi: 10.1309/XFUC-PP6M-5XUA-94B8.
The aim of this study was to determine the efficiency of the Hybrid Capture 2 (HC2; Digene, Gaithersburg, MD) human papillomavirus (HPV) assay for the detection of cervical neoplasia. Of the 1,785 patients recruited, 462 (25.88%) were referred for colposcopy owing to previously detected cytologic abnormalities, and 1,323 (74.12%) were voluntary candidates for screening. For all patients, a Papanicolaou smear and a monolayer smear (ThinPrep, Cytyc, Boxborough, MA) were done. HPV DNA was detected on the residual liquid-based material. False-positive results were observed in 111 cases and comprised 34 cross-reactions (1.90%) and 77 false-positive cases (4.31%) owing to a contiguous strong chemiluminescence signal. Interestingly, all these samples had a relative light units value of 1 to 3 and were contiguous to a sample with a very high HPV DNA load. The final results showed that high-risk and low-risk HPV DNA were detected in 480 samples (26.89%) and 135 samples (7.56%), respectively. Although HC2 can be considered a reliable and sensitive test for HPV DNA detection, we do not advocate its use for large-scale screening for cervical neoplasia.
本研究的目的是确定杂交捕获2代（HC2；Digene公司，马里兰州盖瑟斯堡）人乳头瘤病毒（HPV）检测法在检测宫颈肿瘤方面的效率。在招募的1785名患者中，462名（25.88%）因先前检测到的细胞学异常而被转诊进行阴道镜检查，1323名（74.12%）是自愿参与筛查的对象。对所有患者均进行了巴氏涂片检查和单层涂片检查（ThinPrep，Cytyc公司，马萨诸塞州博克斯伯勒）。在剩余的液基材料上检测HPV DNA。观察到111例假阳性结果，其中包括34例交叉反应（1.90%）和77例假阳性病例（4.31%），原因是相邻的强化学发光信号。有趣的是，所有这些样本的相对光单位值为1至3，且与HPV DNA载量非常高的样本相邻。最终结果显示，分别在480个样本（26.89%）和135个样本（7.56%）中检测到高危和低危HPV DNA。虽然HC2可被认为是一种检测HPV DNA的可靠且灵敏的检测方法，但我们不主张将其用于宫颈肿瘤的大规模筛查。
