Atsuta Yoshiko, Hamajima Nobuyuki
Department of Preventive Medicine/Biostatistics and Medical Decision Making, Nagoya University Graduate School of Medicine, Nagoya 466-8550.
Rinsho Byori. 2003 Sep;51(9):886-91.
The polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is an inexpensive genotyping method, which is applicable for most single nucleotide polymorphisms(SNPs). In this method, allele-specific DNA products are amplified by applying appropriately designed two-pair primers (four primers) into a conventional PCR tube, followed by agarose gel electrophoresis. Duplex or triplex PCR-CTPP is also possible in a single tube, which reduces time and costs. An example of multiplex PCR-CTPP is described for NQO1 C609, GSTM1, and GSTT1. This convenient genotyping tool may be useful in health checkup to detect high risk individuals for some diseases, and to prevent such individuals from developing the diseases.
两对引物对抗聚合酶链反应（PCR-CTPP）是一种成本低廉的基因分型方法，适用于大多数单核苷酸多态性（SNP）。在该方法中，通过将适当设计的两对引物（四条引物）加入常规PCR管中扩增等位基因特异性DNA产物，随后进行琼脂糖凝胶电泳。单管中也可进行双重或三重PCR-CTPP，这可减少时间和成本。文中描述了针对NQO1 C609、GSTM1和GSTT1的多重PCR-CTPP实例。这种便捷的基因分型工具在健康检查中可能有助于检测某些疾病的高危个体，并预防这些个体发病。
