Hamajima N
Division of Epidemiology and Prevention, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan.
Expert Rev Mol Diagn. 2001 May;1(1):119-23. doi: 10.1586/14737159.1.1.119.
A new PCR method, PCR-CTPP (polymerase chain reaction with confronting two-pair primers) was invented to genotype a relatively large number of samples in a cost-effective and time-saving manner. In this method, allele-specific DNA products are amplified by means of applying appropriately designed two-pair primers (four primers) into an ordinary PCR tube. Single genotyping for G2886T at L-myc, Arg72Pro of p53 and Glu487Lys of ALDH2 as well as duplex genotyping for C-31T of IL-1B with VNTR of IL-1RN and A385T of secretor gene with se5, are demonstrated as examples with the primers and PCR conditions.
一种新的PCR方法，即PCR-CTPP（两对引物对向聚合酶链反应）被发明出来，以便以经济高效且节省时间的方式对相对大量的样本进行基因分型。在该方法中，通过将适当设计的两对引物（四条引物）加入普通PCR管中，来扩增等位基因特异性DNA产物。以L-myc基因的G2886T、p53基因的Arg72Pro以及ALDH2基因的Glu487Lys的单基因分型，以及IL-1B基因的C-31T与IL-1RN基因的VNTR、分泌基因的A385T与se5的双重基因分型为例，展示了所用的引物和PCR条件。
