Davies S, Gear J E, Mason C M, McIntyre S M, Hall L
Microbiology Department, Sheffield Teaching Hospitals, Northern General Hospital, Sheffield S5 7AU, UK.
Br J Biomed Sci. 2003;60(3):136-40. doi: 10.1080/09674845.2003.11783689.
This study compares a recently introduced latex agglutination test for the serogrouping of beta-haemolytic streptococci against four internationally used commercial kits. The new kit is Prolex-Blue (Pro-Lab Diagnostics) and the comparators are Streptex (Murex), PathoDx (DPC), Streptococcus Grouping kit (Oxoid) and Prolex-White (Pro-Lab Diagnostics). A total of 302 consecutive clinical isolates are tested against all five kits, following the individual manufacturer's protocol, for both accuracy and speed. In addition, the data produced permits determination of the strengths or weaknesses of the kits against individual serotypes. Prolex-Blue proved to be both accurate and rapid, with a sensitivity of 99% and a specificity of 100%. Furthermore, average time to agglutination was substantially less than achieved by three of the other four kits evaluated.
本研究将一种最近推出的用于β溶血性链球菌血清分组的乳胶凝集试验与四种国际上常用的商业试剂盒进行了比较。新试剂盒是Prolex-Blue（Pro-Lab诊断公司），对照试剂盒是Streptex（Murex公司）、PathoDx（DPC公司）、链球菌分组试剂盒（Oxoid公司）和Prolex-White（Pro-Lab诊断公司）。按照各制造商的方案，对总共302株连续的临床分离株进行了所有五种试剂盒的准确性和速度测试。此外，所产生的数据有助于确定各试剂盒针对不同血清型的优缺点。Prolex-Blue试剂盒被证明既准确又快速，灵敏度为99%，特异性为100%。此外，其平均凝集时间大大短于所评估的其他四种试剂盒中的三种。
