Kalia N, Brown N J, Wood R F M, Hopkinson K, Fairburn B, Pockley A G
Department of Biomedical Science, University of Sheffield, UK.
Dig Dis Sci. 2003 Sep;48(9):1677-84. doi: 10.1023/a:1025418322527.
Peripheral blood neutrophil activation status is indicative of remote organ damage after intestinal ischemia secondary to aortic aneurysm repair. However, the effects of direct intestinal ischemia-reperfusion (I/R) injury on neutrophil activation and its reflection of remote organ injury have not been evaluated. DA rats were subjected to 30 min of intestinal ischemia or sham surgery. Blood samples were taken before ischemia and 30, 60, and 120 min after reperfusion. Neutrophil counts were quantified and CD11b, CD62L, and NKR-P1 expression was assessed using flow cytometry. The sham procedure induced increases in neutrophil numbers (P < 0.001), which was transiently attenuated in animals subjected to intestinal I/R injury. CD11b expression increased in both groups, whereas CD62L and NKR-P1 (P < 0.01) expression decreased in both groups. These findings suggest that even mild surgical procedures induce demargination of neutrophils. Monitoring the peripheral blood for activated neutrophils is of no value in assessing the severity of direct intestinal I/R injury or predicting remote organ damage after intestinal ischemia.
外周血中性粒细胞激活状态可指示主动脉瘤修复后继发性肠缺血后的远隔器官损伤。然而，直接肠缺血-再灌注（I/R）损伤对中性粒细胞激活的影响及其对远隔器官损伤的反映尚未得到评估。将DA大鼠进行30分钟的肠缺血或假手术。在缺血前以及再灌注后30、60和120分钟采集血样。使用流式细胞术对中性粒细胞计数进行定量，并评估CD11b、CD62L和NKR-P1的表达。假手术导致中性粒细胞数量增加（P<0.001），而在遭受肠I/R损伤的动物中这种增加短暂减弱。两组中CD11b表达均增加，而两组中CD62L和NKR-P1（P<0.01）表达均降低。这些发现表明，即使是轻微的外科手术也会诱导中性粒细胞的边缘移动。监测外周血中活化的中性粒细胞对于评估直接肠I/R损伤的严重程度或预测肠缺血后的远隔器官损伤没有价值。
