奥曲肽通过抑制Akt/PKB和端粒酶对胃癌细胞产生抗增殖作用。
Antiproliferative effect of octreotide on gastric cancer cells mediated by inhibition of Akt/PKB and telomerase.
作者信息
Gao Shan, Yu Bao-Ping, Li Yan, Dong Wei-Guo, Luo He-Sheng
机构信息
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
出版信息
World J Gastroenterol. 2003 Oct;9(10):2362-5. doi: 10.3748/wjg.v9.i10.2362.
AIM
To investigate the antiproliferative effect of octreotide, a long-acting analogue of somatostatin, on gastric cancer cell line SGC7901 and its possible molecular mechanisms.
METHODS
Gastric cancer cell line SGC7901 employed in the study was treated with 0.008, 0.04, 0.2, 1, 5 and 25 microg.ml(-1) of octreotide respectively for 24 h to evaluate the antiproliferative effect of somatostatin analog on the tumor cells by MTT assay method. To elucidate the underlying mechanism, the cells were exposed to 1 microg.ml(-1) of octreotide for 0, 12, 24 and 48 h, when their Akt/PKB and telomerase activities were respectively determined using PCR-ELSIA and nonradioactive protein kinase assay protocols. The same experimental procedures were also performed in the control cells that were treated with corresponding vehicles instead of somatostatin analog.
RESULTS
After exposed to octreotide for 24 h at the concentrations of more than 1 microg.ml(-1), SGC7901 cells exhibited a dose-dependent inhibition of growth with the inhibiting rate to be as high as 34.66% when 25 microg.ml(-1) of octreotide was applied. The Akt/PKB and telomerase activity of SGC7901 cells was significantly inhibited when the cells were exposed to 1 microg.ml(-1) of octreotide for 12, 24 and 48 h compared with that of their control counterparts (P<0.01), both of which exhibited in a time-dependent manner.
CONCLUSION
The antiproliferative effect of octreotide on SGC7901 cells might be mediated by the inhibition of Akt/PKB and telomerase.
目的
研究生长抑素长效类似物奥曲肽对胃癌细胞系SGC7901的抗增殖作用及其可能的分子机制。
方法
将研究中使用的胃癌细胞系SGC7901分别用0.008、0.04、0.2、1、5和25μg·ml⁻¹的奥曲肽处理24小时，通过MTT法评估生长抑素类似物对肿瘤细胞的抗增殖作用。为阐明潜在机制，将细胞暴露于1μg·ml⁻¹的奥曲肽中0、12、24和48小时，然后分别使用PCR-ELSIA和非放射性蛋白激酶检测方案测定其Akt/PKB和端粒酶活性。在用相应溶剂而非生长抑素类似物处理的对照细胞中也进行相同的实验步骤。
结果
在浓度超过1μg·ml⁻¹的奥曲肽作用24小时后，SGC7901细胞呈现剂量依赖性生长抑制，当应用25μg·ml⁻¹的奥曲肽时，抑制率高达34.66%。与对照细胞相比，当SGC7901细胞暴露于1μg·ml⁻¹的奥曲肽中12、24和48小时时，其Akt/PKB和端粒酶活性均受到显著抑制（P<0.01），且均呈时间依赖性。
结论
奥曲肽对SGC7901细胞的抗增殖作用可能是通过抑制Akt/PKB和端粒酶介导的。
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