Department of Biology, College of Education for Pure Sciences, University of Kerbala, Kerbala, Iraq.
Arch Razi Inst. 2022 Aug 31;77(4):1389-1395. doi: 10.22092/ARI.2022.357807.2104. eCollection 2022 Aug.
The is a perennial herbaceous plant belonging to the family . Several pharmacological investigations have been performed based on the medicinal application of . The anticancer and antidiabetic activities of fruit and seed extracts of have been studied. Newly developed anticancer/antitumor medications appear to have been developed based on the extracted chemicals from due to the high contents of cucurbitacins. The present study aimed to identify the cytotoxic effect of the crude alcoholic extract of plants of on the growth of human hepatocyte carcinoma (Hep-G2). The results of the chemical (preliminary) examination of the extract indicated that the fruits contain most of the secondary metabolites including Flavonoids, Tannins, Sapiens, Resins, Amino acids, Glycosides, Terpenes, Alkaloids, and Flavonoids. The toxicological effect of the crude extract was investigated by using six half dilutions concentrations of 20,10,5,2.5,1.25, and 0.625 µg/m at three exposure periods of 24,48, and 72 h using MTT testing. The toxicological effect of the extract appeared for all six concentrations in the Hep-G2 cell line. The highest concentration of 20 µg/ml had the highest percentage inhibition rate with a significant difference (≤0.01) and reached 93.36 ±1.61 after 72 h of exposure. While the lowest concentration of 0.625 μg/ml was recorded rate of inhibition of 23.36 ± 2.34 after 24 h of exposure. The findings of the present study concluded that the is one of the most promising medicinal plants which effectively treats cancer through its inhibitory effect and fatal toxicity on cancer cells.
该植物是葫芦科的一种多年生草本植物。已经进行了多项基于 的药用应用的药理学研究。已经研究了果实和种子提取物的抗癌和抗糖尿病活性。似乎已经基于 中提取的化学物质开发了新的抗癌/抗肿瘤药物，因为葫芦素含量很高。本研究旨在确定植物的粗醇提取物对人肝癌（Hep-G2）生长的细胞毒性作用。提取物的化学（初步）检查结果表明，果实中含有大多数次生代谢产物，包括黄酮类、单宁类、三萜类、树脂类、氨基酸类、糖苷类、萜类、生物碱类和黄酮类。通过使用 MTT 测试，在 24、48 和 72 h 的三个暴露期内，用六个半稀释浓度的 20、10、5、2.5、1.25 和 0.625 µg/ml 研究粗提取物的毒性作用。提取物在 Hep-G2 细胞系中对所有六个浓度都表现出毒性作用。最高浓度 20 µg/ml 的抑制率最高，差异有统计学意义（≤0.01），暴露 72 h 后达到 93.36±1.61%。而最低浓度 0.625 μg/ml 的抑制率为 23.36±2.34%，暴露 24 h 后。本研究的结果表明， 是最有前途的药用植物之一，通过其对癌细胞的抑制作用和致命毒性有效治疗癌症。
