单量子点追踪揭示甘氨酸受体的扩散动力学

Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking.

作者信息

Dahan Maxime, Lévi Sabine, Luccardini Camilla, Rostaing Philippe, Riveau Béatrice, Triller Antoine

机构信息

Laboratoire Kastler Brossel, CNRS UMR 8552, Ecole Normale Supérieure and Université Pierre et Marie Curie, 24 rue Lhomond, 75005 Paris, France.

出版信息

Science. 2003 Oct 17;302(5644):442-5. doi: 10.1126/science.1088525.

DOI:10.1126/science.1088525

PMID:14564008

Abstract

Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors.

摘要

半导体量子点（QDs）是适用于先进生物成像的纳米级荧光探针。我们使用量子点来追踪单个甘氨酸受体（GlyRs），并分析它们在活细胞神经元膜中的横向动力学，时间范围从毫秒到分钟。我们表征了与突触、突触周围或突触外甘氨酸受体定位相关的多个扩散域。通过扩散观察到甘氨酸受体进入突触，并通过量子点标记受体的电子显微镜成像进一步证实。