Danial John S H
Centre of Biophotonics, University of St Andrews, St Andrews, UK.
SUPA School of Physics and Astronomy, University of St Andrews, St Andrews, UK.
Nat Methods. 2025 Jun 27. doi: 10.1038/s41592-025-02731-1.
Super-resolution microscopy (SRM) has revolutionized biological imaging, enabling the visualization of biological structures at molecular resolution. However, the diversity of SRM techniques, each operating through different mechanisms, combined with the lack of a clear consensus on the definition of resolution, has hindered the biological community from fully recognizing SRM's contribution to structural biology. I propose a framework for defining, measuring and reporting resolution in SRM. Within this framework, I examine the capabilities of state-of-the-art methods that can achieve 'ångström-scale resolution' and clarify the level of structural detail users can expect to observe. Finally, drawing from recent advancements, I explore pathways to extend SRM towards live structural imaging.
超分辨率显微镜（SRM）彻底改变了生物成像技术，能够以分子分辨率观察生物结构。然而，SRM技术的多样性，每种技术都通过不同的机制运行，再加上对分辨率定义缺乏明确的共识，阻碍了生物学界充分认识SRM对结构生物学的贡献。我提出了一个用于定义、测量和报告SRM分辨率的框架。在这个框架内，我研究了能够实现“埃级分辨率”的最先进方法的能力，并阐明了用户可以期望观察到的结构细节水平。最后，借鉴最近的进展，我探索了将SRM扩展到实时结构成像的途径。
