Anderson G M, Hall L M, Yang J X, Cohen D J
Child Study Center, Yale University School of Medicine, New Haven, Connecticut 06510.
Anal Biochem. 1992 Oct;206(1):64-7. doi: 10.1016/s0003-2697(05)80011-9.
The platelet dense granule release reaction was monitored by measuring the serotonin content of platelets collected by filtration following release. Sensitive HPLC-fluorometric analysis permitted release to be assessed using small (25-50 microliters) volumes of plasma and whole blood. Release could be examined for periods as short as 1 s due to the rapid stoppage effected by filtration. The half-life of thrombin-stimulated (1 U/ml) release was 2-3 s, the EC50 values observed for thrombin-stimulated release over a 60-s release period were 0.01-0.03 U/ml for diluted whole blood and plasma samples.
通过测量释放后经过滤收集的血小板中5-羟色胺的含量来监测血小板致密颗粒释放反应。灵敏的高效液相色谱-荧光分析允许使用少量（25-50微升）血浆和全血来评估释放情况。由于过滤实现的快速阻断，可在短至1秒的时间段内检查释放情况。凝血酶刺激（1 U/ml）释放的半衰期为2-3秒，在60秒释放期内凝血酶刺激稀释全血和血浆样品释放所观察到的EC50值为0.01-0.03 U/ml。
