[采用荧光检测高效液相色谱法测定全血和富血小板血浆中的血清素]

[Determination of serotonin in whole blood and platelet rich plasma by high-performance liquid chromatography with fluorometric detection].

作者信息

Oka T, Kobayashi K, Hirauchi K, Igimi H, Uchida K

机构信息

Diagnostic Science Department, Shionogi & Co., Ltd., Settsu.

出版信息

Rinsho Byori. 1994 Jul;42(7):748-52.

PMID:8065043

Abstract

An improved high-performance liquid chromatographic method with fluorometric detection (Ex 285nm, Em 345nm) for the assay of serotonin in whole blood and platelet rich plasma was developed for the routine clinical examination. Clinical sample was successfully deproteinized with perchloric acid in the presence of a reducing agent (ascorbic acid). Precision (CV < 5.0%), accuracy (recovery > 95%), lower assay limit (20ng/ml) and assay time (15min/sample) were allowable for the routine clinical examination. The level of serotonin (ng/ml, mean +/- SD) in whole blood and platelet rich plasma from normal volunteers (n = 53) was 143 +/- 47 and 180 +/- 60, respectively.

摘要

建立了一种改进的高效液相色谱法，采用荧光检测（激发波长285nm，发射波长345nm）测定全血和富血小板血浆中的血清素，用于常规临床检查。临床样本在还原剂（抗坏血酸）存在下用高氯酸成功脱蛋白。精密度（CV<5.0%）、准确度（回收率>95%）、较低检测限（20ng/ml）和检测时间（15分钟/样本）对于常规临床检查是可以接受的。正常志愿者（n=53）全血和富血小板血浆中血清素水平（ng/ml，平均值±标准差）分别为143±47和180±60。