Chutmongkonkul M, Maier W A, Seitz H M
Institut für Medizinische Parasitologie, Universität Bonn.
Angew Parasitol. 1992 Nov;33(4):217-25.
The aim of this study was to find optimal conditions for the membrane feeding technique to obtain maximum infection rates of mosquitoes with Plasmodium yoelii nigeriensis. The results show that the malaria parasite Plasmodium yoelii nigeriensis is most infective to Anopheles stephensi mosquitoes on day 3 of the infection in the mice, 1 day before the peak of parasitaemia. The mortality rate of the mosquitoes fed on mice on day 3 after infection was the highest as compared to mosquitoes fed on other days after infection. Gametocytes from mice 3 days after infection were fed to mosquitoes by three different membrane feeding methods. The results indicate that feeding during the first 10 min after blood collection gave the highest infection rates. Keeping the blood meal at a pH of 7.2 yields higher infection rates than keeping it at pH of 8.5. Stirring of the blood and supplying it with CO2 is not necessary when feeding of the mosquitoes is completed within the first 10 min after collection of the blood.
本研究的目的是找到膜饲技术的最佳条件，以获得约氏疟原虫尼日尔株对蚊子的最高感染率。结果表明，疟原虫约氏疟原虫尼日尔株在小鼠感染后第3天，即寄生虫血症高峰前1天，对斯氏按蚊的感染性最强。与感染后其他日子喂食的蚊子相比，感染后第3天喂食小鼠的蚊子死亡率最高。通过三种不同的膜饲方法，将感染后3天小鼠的配子体喂给蚊子。结果表明，采血后前10分钟内喂食感染率最高。血餐pH值保持在7.2时的感染率高于pH值保持在8.5时。如果在采血后前10分钟内完成蚊子喂食，则无需搅拌血液并供应二氧化碳。
