Chivot L, Maillot-Vest M, Mainard F, Pare J M, Massoubre C, Madec Y
Laboratoire de biochimie A, CHU Hôtel-Dieu, Nantes, France.
Ann Biol Clin (Paris). 1992;50(3):143-7.
Latex immunoturbidimetric (ITA) and immunonephelemetric (INA) assays, were evaluated and compared to a radioimmunological assay (RIA) for the determination of serum myoglobin. Within-run and between-run assays and accuracy and linearity studies gave satisfactory results, very similar to those of RIA. Results were closely correlated with those of RIA for 100 samples of up to 2,000 micrograms/l (correlation coefficients respectively 0.96 and 0.97 for ITA and INA). Reference values (150 men and 150 women; range 20-65 years) were determined and expressed in percentiles. It is concluded that the two latex immunologic assays facilitate laboratory myoglobin evaluation (no radioactive material, short performance time). ITA is more suitable for emergency analysis and INA is more convenient for serial analysis and particular samples (presence of hemoglobin or chylomicrons).
对乳胶免疫比浊法（ITA）和免疫散射比浊法（INA）进行了评估，并与放射免疫分析法（RIA）进行比较，以测定血清肌红蛋白。批内和批间分析以及准确性和线性研究均得出了令人满意的结果，与放射免疫分析法的结果非常相似。对100份浓度高达2000微克/升的样本进行检测，结果与放射免疫分析法密切相关（ITA和INA的相关系数分别为0.96和0.97）。确定了参考值（150名男性和150名女性；年龄范围20 - 65岁）并以百分位数表示。得出的结论是，这两种乳胶免疫分析法有助于实验室进行肌红蛋白评估（无放射性物质，检测时间短）。ITA更适合急诊分析，而INA更便于进行系列分析和特殊样本（存在血红蛋白或乳糜微粒）的分析。
