Uji Y, Okabe H, Sugiuchi H, Sekine S
Department of Laboratory Medicine, Kumamoto University Medical School, Japan.
J Clin Lab Anal. 1992;6(1):7-11. doi: 10.1002/jcla.1860060103.
We evaluated an immunoturbidimetric quantitation for serum myoglobin by the latex agglutination method using an automated biochemical analyzer. This method is rapid, specific, accurate, precise, and has wide dynamic range. The total assay time is 10 min and is performed at 37 degrees C with continuous monitoring at 570 nm. The assay results were compared with radioisotopic immunoassay results and showed a good correlation coefficient, r = 0.99; Y = 0.98 x + 9.3; N = 79. Sera from healthy adults has a myoglobin concentration in the range of 15-80 ng/ml(N = 362). Sex- and age-related differences were observed. The serum myoglobin levels in males and elderly people showed higher concentration than in females and younger people. The peak elevation of serum myoglobin compared with other cardiac markers was observed within 6 hours after onset of chest pain as well as the CK-isoform ratio (MM3/MM1). All of the serum from 21 patients with definite acute myocardial infarction showed increased serum myoglobin levels (100-1200 ng/ml) upon admission and within 6 hours. The results suggest that assays for serum myoglobin levels are helpful in the early diagnosis of acute myocardial necrosis.
我们使用自动生化分析仪，通过乳胶凝集法对血清肌红蛋白进行了免疫比浊定量分析。该方法快速、特异、准确、精密，且具有较宽的动态范围。总检测时间为10分钟，在37摄氏度下进行，于570纳米处连续监测。将检测结果与放射性同位素免疫分析结果进行比较，显示出良好的相关系数，r = 0.99；Y = 0.98x + 9.3；N = 79。健康成年人血清肌红蛋白浓度范围为15 - 80纳克/毫升（N = 362）。观察到了性别和年龄相关差异。男性和老年人的血清肌红蛋白水平高于女性和年轻人。与其他心脏标志物相比，血清肌红蛋白在胸痛发作后6小时内达到峰值升高，肌酸激酶同工酶比值（MM3/MM1）也是如此。21例确诊为急性心肌梗死的患者入院时及6小时内的所有血清样本，血清肌红蛋白水平均升高（100 - 1200纳克/毫升）。结果表明，检测血清肌红蛋白水平有助于急性心肌坏死的早期诊断。
