Song Quanlai, Wang Zhiwei, Sanghvi Yogesh S
Isis Pharmaceuticals, Inc., Carlsbad, California 92008, USA.
Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):629-33. doi: 10.1081/NCN-120021968.
Dimethylthiuram disulfide (DTD) has been developed as an efficient thiolation reagent during automated synthesis of oligonucleotides using phosphoramidite chemistry. Simultaneous thiolation and capping was accomplished by mixing DTD with capping solution B, which saved 20% of solvent consumption and compressed the four-step synthesis cycle to three. Large-scale (1 mmol) synthesis of phosphorothioate oligonucleotides has been demonstrated with excellent yield and purity.
二硫化二甲基秋兰姆（DTD）已被开发为一种高效的硫醇化试剂，用于在使用亚磷酰胺化学法自动合成寡核苷酸的过程中。通过将DTD与封端溶液B混合实现了同时硫醇化和封端，这节省了20%的溶剂消耗，并将四步合成周期压缩为三步。已证明大规模（1 mmol）合成硫代磷酸寡核苷酸具有优异的产率和纯度。
