Brotschi Christine, Leumann Christian J
Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland.
Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1195-7. doi: 10.1081/NCN-120022834.
The base modified nucleoside dBP, carrying a non-hydrogen-bonding non-shape complementary base was incorporated into oligonucleotides (Brotschi, C.; Häberli, A.; Leumann C.J. Angew. Chem. Int. Ed. 2001, 40, 3012-3014). This base was designed to coordinate transition metal ions into well defined positions within a DNA double helix. Melting experiments revealed that the stability of a dBP:dBP base couple in a DNA duplex is similar to a dG:dC base pair even in the absence of transition metal ions. In the presence of transition metal ions, melting experiments revealed a decrease in duplex stability which is on a similar order for all metal ions (Mn2+, Cu2+, Zn2+, Ni2+) tested.
携带非氢键结合且非形状互补碱基的碱基修饰核苷dBP被掺入寡核苷酸中（布罗茨基，C.；哈伯利，A.；勒曼，C.J.《德国应用化学》国际版2001年，40卷，3012 - 3014页）。该碱基旨在将过渡金属离子配位到DNA双螺旋内明确的位置。熔解实验表明，即使在没有过渡金属离子的情况下，DNA双链体中dBP:dBP碱基对的稳定性与dG:dC碱基对相似。在有过渡金属离子存在的情况下，熔解实验表明双链体稳定性降低，对于所有测试的金属离子（Mn²⁺、Cu²⁺、Zn²⁺、Ni²⁺），降低程度处于相似水平。
