Sugiyama Kumiko, Ono Akira
Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama, Kanagawa 221-8686, Japan.
Nucleic Acids Symp Ser (Oxf). 2006(50):23-4. doi: 10.1093/nass/nrl012.
DNA duplexes containing 4-N-carboxymethylcytosine (1) were synthesized: duplex-I (5'-GTGACCA1TGCAG TG-3':3'-CACTGGT1ACGTCAC-5'), duplex-II (5'-GC CCA1TCCA1TGCG-3':3'-CG GGT1AGGT1ACGC-5'), and duplex-III (5'-GCTCCA11TGCACCG-3':3'-CGAG GT11ACGTGGC-5'). In the presence of Cu(II) or Ni(II) ions, duplex-I and duplex-II, which contain isolated 1-1 pair(s), were stabilized. In contrast, duplex-III, which contained consecutive 1-1 pairs, was stabilized significantly in the presence of Cu(II) ions, while the addition of Ni(II) ions had no notable effect on its thermal denaturation profile.
合成了含有4-N-羧甲基胞嘧啶（1）的DNA双链体：双链体-I（5'-GTGACCA1TGCAG TG-3'：3'-CACTGGT1ACGTCAC-5'）、双链体-II（5'-GC CCA1TCCA1TGCG-3'：3'-CG GGT1AGGT1ACGC-5'）和双链体-III（5'-GCTCCA11TGCACCG-3'：3'-CGAG GT11ACGTGGC-5'）。在铜（II）或镍（II）离子存在下，含有孤立的1-1碱基对的双链体-I和双链体-II得到稳定。相比之下，含有连续1-1碱基对的双链体-III在铜（II）离子存在下显著稳定，而添加镍（II）离子对其热变性曲线没有显著影响。
