Synthesis and MS analysis of thiazolium and pyridinium derivatives of peptide nucleic acids (PNAs) and peptides.
作者信息
Kovács G, Pádár P, Kupihár Z, Kele Z, Forgó P, Kovács L
机构信息
Department of Medicinal Chemistry, Nucleic Acids Laboratory, University of Szeged, Szeged, Hungary.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1301-3. doi: 10.1081/NCN-120022951.
PMID:14565404
Abstract
Sensitivity of ESI-MS analysis of crude PNAs is enhanced using their pyridinium or thiazolium derivatives. Identification of the molecular ion of the product is easier when the label contains bromine, based on the isotope distribution. Study of side reactions, occurred upon the synthesis and/or cleavage, is simple with labelling. Sequencing of non-polar peptides is clear as only a(n) type ions can be observed during their MS/MS analysis.
摘要
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