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Understanding high diastereomeric discrimination in formation of oligoribonucleotide phosphorothioate linkages: the first study of pKa-dependent activation in solid-supported coupling of 2'-O-substituted ribonucleoside phosphoramidites.

作者信息

Ravikumar Vasulinga T, Cole Douglas L

机构信息

Isis Pharmaceuticals, Carlsbad, California 92009, USA.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1415-9. doi: 10.1081/NCN-120022999.

DOI:10.1081/NCN-120022999
PMID:14565432
Abstract

Activation of 2'-O-substituted ribonucleoside phosphoramidites with various activators during solid-supported synthesis of phosphorothioate oligonucleotides was studied. The Rp:Sp diastereomeric composition of resulting phosphorothioate linkage dependent on pKa of activator utilized for coupling.

摘要

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