通过使用2-（乙酰氧基甲基）-5-硝基苯甲酰基（LMNBZ）基团对核糖核苷和2'-脱氧核糖核苷3'-亚磷酰胺的5'-羟基进行寡核苷酸合成。
Oligonucleotide synthesis by the use of a 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBZ) group for the 5'-hydroxyl group of ribonucleoside and 2'-deoxyribonucleoside 3'-phosphoramidites.
作者信息
Kamaike K, Takahashi H, Kakinuma T, Morohoshi K, Ishido Y
机构信息
Laboratory of Pharmaceutical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
出版信息
Nucleic Acids Symp Ser. 1997(37):45-6.
PMID:9585991
Abstract
Synthetic studies on the CpCpA terminus triplet, whose exocyclic amino groups are all labeled with 15N, and a phosphorodithioate-linked oligonucleotide in terms of 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) group as the novel base-labile protecting group for the 5'-hydroxyl groups of nucleoside 3'-phosphoramidites, are described.
摘要
对CpCpA末端三联体（其环外氨基均用15N标记）以及一种硫代磷酸酯连接的寡核苷酸进行了合成研究，该研究涉及以2-(乙酰氧基甲基)-5-硝基苯甲酰基（LMNBz）基团作为核苷3'-磷酰胺酯5'-羟基的新型碱不稳定保护基。
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