Ng Pei-Sze, Pingle Maneesh R, Balasundarum Ganesan, Friedman Alan, Zu Xiaolin, Bergstrom Donald E
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907-1333, USA.
Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1635-7. doi: 10.1081/NCN-120023088.
The syntheses of endcaps for covalently linking the 3' and 5' hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the endcaps on duplex DNA was determined by Tm experiments on the self-complementary octamer.
