Zhang Di, Li Yamin, Xu Liang, Cheng Maosheng, Zhou Ying, He Junlin, Liu Keliang
School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, P. R. China.
Nucleosides Nucleotides Nucleic Acids. 2010 Oct;29(10):734-47. doi: 10.1080/15257770.2010.510124.
Tert-butyldiphenylsilyl (TBDPS) was testified to be an appropriate orthogonal protecting group for novel 7-hydroxyl-functionalized 8-aza-7-deaza-2'-deoxyadenosine analogues. It was stable in partial and complete hydrogenation reactions used for the different linker preparation. The corresponding phosphoramidites and hydroxyl-functionalized oligodeoxynucleotides were synthesized and identified. The thermal effect of the hydroxyl group with different linkers on DNA duplexes was evaluated. It provided a feasible strategy for the preparation of hydroxyl-functionalized DNAs for the nucleic acid research.
