一种与肯德林和CG-NAP具有同源性的果蝇中心体蛋白CP309的特性分析。
Characterization of a Drosophila centrosome protein CP309 that shares homology with Kendrin and CG-NAP.
作者信息
Kawaguchi Shin-ichi, Zheng Yixian
机构信息
Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, Baltimore, Maryland 21210, USA.
出版信息
Mol Biol Cell. 2004 Jan;15(1):37-45. doi: 10.1091/mbc.e03-03-0191. Epub 2003 Oct 17.
Abstract
The centrosome in animal cells provides a major microtubule-nucleating site that regulates the microtubule cytoskeleton temporally and spatially throughout the cell cycle. We report the identification in Drosophila melanogaster of a large coiled-coil centrosome protein that can bind to calmodulin. Biochemical studies reveal that this novel Drosophila centrosome protein, centrosome protein of 309 kDa (CP309), cofractionates with the gamma-tubulin ring complex and the centrosome-complementing activity. We show that CP309 is required for microtubule nucleation mediated by centrosomes and that it interacts with the gamma-tubulin small complex. These findings suggest that the microtubule-nucleating activity of the centrosome requires the function of CP309.
摘要
动物细胞中的中心体提供了一个主要的微管成核位点，在整个细胞周期中对微管细胞骨架进行时空调节。我们报告了在黑腹果蝇中鉴定出一种能与钙调蛋白结合的大型卷曲螺旋中心体蛋白。生化研究表明，这种新的果蝇中心体蛋白，即309 kDa的中心体蛋白（CP309），与γ-微管蛋白环复合物及中心体互补活性共分离。我们发现CP309是中心体介导的微管成核所必需的，并且它与γ-微管蛋白小复合物相互作用。这些发现表明中心体的微管成核活性需要CP309的功能。
相似文献
1
Characterization of a Drosophila centrosome protein CP309 that shares homology with Kendrin and CG-NAP.一种与肯德林和CG-NAP具有同源性的果蝇中心体蛋白CP309的特性分析。
Mol Biol Cell. 2004 Jan;15(1):37-45. doi: 10.1091/mbc.e03-03-0191. Epub 2003 Oct 17.
2
Centrosomal proteins CG-NAP and kendrin provide microtubule nucleation sites by anchoring gamma-tubulin ring complex.中心体蛋白CG-NAP和肯德林通过锚定γ-微管蛋白环复合物提供微管成核位点。
Mol Biol Cell. 2002 Sep;13(9):3235-45. doi: 10.1091/mbc.e02-02-0112.
3
Identification of a human centrosomal calmodulin-binding protein that shares homology with pericentrin.一种与中心粒外周蛋白具有同源性的人类中心体钙调蛋白结合蛋白的鉴定。
Proc Natl Acad Sci U S A. 2000 May 23;97(11):5919-23. doi: 10.1073/pnas.97.11.5919.
4
Kendrin/pericentrin-B, a centrosome protein with homology to pericentrin that complexes with PCM-1.肯德林/中心体蛋白B，一种与中心体蛋白具有同源性的中心体蛋白，可与PCM-1形成复合物。
J Cell Sci. 2001 Feb;114(Pt 4):797-809. doi: 10.1242/jcs.114.4.797.
5
Proper recruitment of gamma-tubulin and D-TACC/Msps to embryonic Drosophila centrosomes requires Centrosomin Motif 1.γ-微管蛋白和D-TACC/Msps正确定位于胚胎期果蝇中心体需要中心体蛋白基序1。
Mol Biol Cell. 2007 Oct;18(10):4037-49. doi: 10.1091/mbc.e07-05-0474. Epub 2007 Aug 1.
6
Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits.果蝇γ-微管蛋白环复合物亚基的表征与重组
J Cell Biol. 2000 Dec 25;151(7):1513-24. doi: 10.1083/jcb.151.7.1513.
7
Interphase centrosome organization by the PLP-Cnn scaffold is required for centrosome function.中心体功能需要由PLP-Cnn支架进行的间期中心体组织。
J Cell Biol. 2015 Jul 6;210(1):79-97. doi: 10.1083/jcb.201503117.
8
Distinct Dgrip84 isoforms correlate with distinct gamma-tubulins in Drosophila.在果蝇中，不同的Dgrip84同工型与不同的γ-微管蛋白相关。
Mol Biol Cell. 2008 Jan;19(1):368-77. doi: 10.1091/mbc.e07-08-0801. Epub 2007 Nov 14.
9
Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle.Augmin：一种纺锤体内不依赖中心体的微管生成所需的蛋白质复合体。
J Cell Biol. 2008 May 5;181(3):421-9. doi: 10.1083/jcb.200711053. Epub 2008 Apr 28.
10
Cortical actin dynamics facilitate early-stage centrosome separation.皮层肌动蛋白动力学促进早期中心体分离。
Curr Biol. 2010 Apr 27;20(8):770-6. doi: 10.1016/j.cub.2010.02.060. Epub 2010 Apr 22.
引用本文的文献
1
The Impact of Centrosome Pathologies on Ovarian Cancer Development and Progression with a Focus on Centrosomes as Therapeutic Target.中心体病变对卵巢癌发生发展的影响及其作为治疗靶点的研究进展。
Adv Exp Med Biol. 2024;1452:37-64. doi: 10.1007/978-3-031-58311-7_3.
2
Sperm Head-Tail Linkage Requires Restriction of Pericentriolar Material to the Proximal Centriole End.精子头-尾连接需要将中心粒周围物质限制在近端中心粒末端。
Dev Cell. 2020 Apr 6;53(1):86-101.e7. doi: 10.1016/j.devcel.2020.02.006. Epub 2020 Mar 12.
3
Mitotic Spindle Assembly in Land Plants: Molecules and Mechanisms.陆地植物中的有丝分裂纺锤体组装：分子与机制
Biology (Basel). 2017 Jan 25;6(1):6. doi: 10.3390/biology6010006.
4
Klp10A modulates the localization of centriole-associated proteins during Drosophila male gametogenesis.Klp10A在果蝇雄性配子发生过程中调节中心粒相关蛋白的定位。
Cell Cycle. 2016 Dec 16;15(24):3432-3441. doi: 10.1080/15384101.2016.1248005. Epub 2016 Oct 20.
5
The Drosophila Pericentrin-like-protein (PLP) cooperates with Cnn to maintain the integrity of the outer PCM.果蝇类中心体蛋白（PLP）与 Cnn 合作，维持外层 PCM 的完整性。
Biol Open. 2015 Jul 8;4(8):1052-61. doi: 10.1242/bio.012914.
6
Interphase centrosome organization by the PLP-Cnn scaffold is required for centrosome function.中心体功能需要由PLP-Cnn支架进行的间期中心体组织。
J Cell Biol. 2015 Jul 6;210(1):79-97. doi: 10.1083/jcb.201503117.
7
A molecular mechanism of mitotic centrosome assembly in Drosophila.果蝇有丝分裂中心体组装的分子机制。
Elife. 2014 Aug 22;3:e03399. doi: 10.7554/eLife.03399.
8
When fate follows age: unequal centrosomes in asymmetric cell division.当命运随年龄而定：不对称细胞分裂中不等的中心体
Philos Trans R Soc Lond B Biol Sci. 2014 Sep 5;369(1650). doi: 10.1098/rstb.2013.0466.
9
The centrosome-Golgi apparatus nexus.中心体-高尔基体联系
Philos Trans R Soc Lond B Biol Sci. 2014 Sep 5;369(1650). doi: 10.1098/rstb.2013.0462.
10
Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies.果蝇中心体蛋白在中心体和神经元基体发挥功能时需要与钙调蛋白相互作用，但在精子基体中则不需要。
Mol Biol Cell. 2014 Sep 15;25(18):2682-94. doi: 10.1091/mbc.E13-10-0617. Epub 2014 Jul 16.
本文引用的文献
1
Characterization of a new gammaTuRC subunit with WD repeats.具有WD重复序列的新型γ微管蛋白环形复合物亚基的鉴定
Mol Biol Cell. 2003 Mar;14(3):1017-26. doi: 10.1091/mbc.e02-01-0034.
2
Centrosomal proteins CG-NAP and kendrin provide microtubule nucleation sites by anchoring gamma-tubulin ring complex.中心体蛋白CG-NAP和肯德林通过锚定γ-微管蛋白环复合物提供微管成核位点。
Mol Biol Cell. 2002 Sep;13(9):3235-45. doi: 10.1091/mbc.e02-02-0112.
3
Centrosome composition and microtubule anchoring mechanisms.中心体组成与微管锚定机制。
Curr Opin Cell Biol. 2002 Feb;14(1):25-34. doi: 10.1016/s0955-0674(01)00290-3.
4
GCP5 and GCP6: two new members of the human gamma-tubulin complex.GCP5和GCP6：人γ-微管蛋白复合体的两个新成员。
Mol Biol Cell. 2001 Nov;12(11):3340-52. doi: 10.1091/mbc.12.11.3340.
5
Purification and reconstitution of Drosophila gamma-tubulin complexes.果蝇γ-微管蛋白复合体的纯化与重组
Methods Cell Biol. 2001;67:1-25. doi: 10.1016/s0091-679x(01)67002-x.
6
Outer dense fiber 2 is a widespread centrosome scaffold component preferentially associated with mother centrioles: its identification from isolated centrosomes.外致密纤维2是一种广泛存在的中心体支架成分，优先与母中心粒相关联：从分离的中心体中对其进行鉴定。
Mol Biol Cell. 2001 Jun;12(6):1687-97. doi: 10.1091/mbc.12.6.1687.
7
The PACT domain, a conserved centrosomal targeting motif in the coiled-coil proteins AKAP450 and pericentrin.PACT结构域，一种存在于卷曲螺旋蛋白AKAP450和中心体蛋白中的保守的中心体靶向基序。
EMBO Rep. 2000 Dec;1(6):524-9. doi: 10.1093/embo-reports/kvd105.
8
Kendrin/pericentrin-B, a centrosome protein with homology to pericentrin that complexes with PCM-1.肯德林/中心体蛋白B，一种与中心体蛋白具有同源性的中心体蛋白，可与PCM-1形成复合物。
J Cell Sci. 2001 Feb;114(Pt 4):797-809. doi: 10.1242/jcs.114.4.797.
9
The role of Xgrip210 in gamma-tubulin ring complex assembly and centrosome recruitment.Xgrip210在γ-微管蛋白环复合物组装和中心体招募中的作用。
J Cell Biol. 2000 Dec 25;151(7):1525-36. doi: 10.1083/jcb.151.7.1525.
10
Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits.果蝇γ-微管蛋白环复合物亚基的表征与重组
J Cell Biol. 2000 Dec 25;151(7):1513-24. doi: 10.1083/jcb.151.7.1513.
Zotero 插件