Cell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Cell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Dev Cell. 2020 Apr 6;53(1):86-101.e7. doi: 10.1016/j.devcel.2020.02.006. Epub 2020 Mar 12.
The centriole, or basal body, is the center of attachment between the sperm head and tail. While the distal end of the centriole templates the cilia, the proximal end associates with the nucleus. Using Drosophila, we identify a centriole-centric mechanism that ensures proper proximal end docking to the nucleus. This mechanism relies on the restriction of pericentrin-like protein (PLP) and the pericentriolar material (PCM) to the proximal end of the centriole. PLP is restricted proximally by limiting its mRNA and protein to the earliest stages of centriole elongation. Ectopic positioning of PLP to more distal portions of the centriole is sufficient to redistribute PCM and microtubules along the entire centriole length. This results in erroneous, lateral centriole docking to the nucleus, leading to spermatid decapitation as a result of a failure to form a stable head-tail linkage.
中心粒或基体是精子头部和尾部之间的附着中心。当中心粒的远端模板化纤毛时，近端与核相连。我们利用果蝇鉴定了一种以中心粒为中心的机制，该机制确保了近端正确地与核对接。该机制依赖于中心粒蛋白（PLP）和中心粒周围物质（PCM）限制在中心粒的近端。PLP 的限制是通过将其 mRNA 和蛋白质限制在中心粒伸长的最早阶段来实现的。将 PLP 异位定位到中心粒的更远端足以沿整个中心粒长度重新分配 PCM 和微管。这导致错误的侧部中心粒与核对接，导致精子头部脱落，因为无法形成稳定的头部-尾部连接。
