Peng Xiao-Li, Gao Xi-Ling, Chen Jun, Huang Xi, Chen Hui-Sheng
Pain Research Center, Institute of Neuroscience, The Fourth Military Medical University, Xi' an 710032.
Sheng Li Xue Bao. 2003 Oct 25;55(5):516-24.
To study whether commercial traditional Chinese medicinal preparations Injection Paederiae (IP) or Injection Stauntonia (IS) has anti-nociceptive and/or anti-inflammatory effects, we used two persistent pain models (bee venom and formalin test) to evaluate the systemic effects of IP or IS on the chemical tissue injury-induced persistent spontaneous pain-related responses (PSPR), primary thermal/mechanical hyperalgesia and inflammation in conscious rats. Injection of bee venom (BV, 0.1 mg, 50 microl) into the plantar surface of one hind paw resulted in not only a 1-h monophasic PSPR such as flinching reflex in the injected paw and a subsequent period of 3-4 days primary heat and mechanical hyperalgesia, but also a marked sign of inflammation, including redness and swelling of the plantar surface in the injected paw. Intraplantar injection of formalin produced two phases of PSPR as reported previously. Systemic pre-treatment with three doses of IP (0.32, 1.6 and 9.0 ml/kg, 500%) or IS (0.32, 1.6 and 9.0 ml/kg, 250%) produced a dose-dependent suppression of the BV- or formalin-induced flinching reflex of 1 h time course as compared with the saline control group. Post-treatment with IP or IS 5 min after BV injection also produced a significant suppression of the flinching reflex in both BV test and formalin test respectively, as compared with the control group. However, neither pre- nor post-treatment with IP or IS produced any significantly suppressive effect on the BV-induced primary heat and mechanical hyperalgesia and inflammation. The analgesia produced by IP or IS was not mediated by the endogenous opioid receptors since naloxone, a non-selective opioid receptor antagonist, had no reversal effect on the IP and IS-produced analgesia in the BV-induced PSPR. Our present results suggest that IP or IS might prevent and relieve clinical persistent spontaneous pain, but without any anti-nociceptive and anti-inflammatory effects on the primary heat hyperalgesia, mechanical hyperalgesia, as well as inflammatory responses. The BV test might be a useful model of pain to evaluate and screen anti-nociceptive and anti-inflammatory effects of certain compounds of the Chinese medicinal herbs on the pathological origins of pain.
为研究市售中药制剂肿节风注射液（IP）或野木瓜注射液（IS）是否具有抗伤害性感受和/或抗炎作用，我们采用两种持续性疼痛模型（蜂毒和福尔马林试验）来评估IP或IS对清醒大鼠化学性组织损伤诱导的持续性自发疼痛相关反应（PSPR）、原发性热/机械性痛觉过敏及炎症的全身影响。将蜂毒（BV，0.1mg，50μl）注射到一只后爪的足底表面，不仅会导致1小时的单相PSPR，如注射爪的退缩反射以及随后3 - 4天的原发性热和机械性痛觉过敏，还会出现明显的炎症迹象，包括注射爪足底表面的发红和肿胀。如先前报道，足底注射福尔马林会产生两个阶段的PSPR。与生理盐水对照组相比，用三剂IP（0.32、1.6和9.0ml/kg，500%）或IS（0.32、1.6和9.0ml/kg，250%）进行全身预处理可剂量依赖性地抑制BV或福尔马林诱导的1小时时程的退缩反射。在BV注射后5分钟用IP或IS进行后处理，与对照组相比，在BV试验和福尔马林试验中也分别显著抑制了退缩反射。然而，IP或IS的预处理和后处理均未对BV诱导的原发性热和机械性痛觉过敏及炎症产生任何显著的抑制作用。IP或IS产生的镇痛作用不是由内源性阿片受体介导的，因为非选择性阿片受体拮抗剂纳洛酮对BV诱导的PSPR中IP和IS产生的镇痛作用没有逆转作用。我们目前的结果表明，IP或IS可能预防和缓解临床持续性自发疼痛，但对原发性热痛觉过敏、机械性痛觉过敏以及炎症反应没有任何抗伤害性感受和抗炎作用。BV试验可能是一种有用的疼痛模型，用于评估和筛选中药某些化合物对疼痛病理起源的抗伤害性感受和抗炎作用。
