Wu Xiao-Jing, Huang Lan, Song Dai-Liang, Jin Jun, Zhao Gang
The Cardiologic Center of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, PR China.
Sheng Li Xue Bao. 2003 Oct 25;55(5):554-9.
Endothelial injury, smooth muscle cells (SMCs) proliferation and migration are the same common pathophysiological processes of many cardiovascular diseases, such as atherosclerosis, hypertension, diabetes and restenosis. It is important to determine the functional interactions between endothelial cells (ECs) and SMCs under pathologic conditions. This work was to study the effects of ECs growth states on the proliferation and migration of vascular SMCs in cell coculture system. (3)H-TdR incorporation and flow cytometry were used to determine the effects of ECs growth states on the proliferation of SMCs. The number of migrating SMCs was counted. RT-PCR was used to analyze the expression of alpha-SM-actin mRNA. The results showed that (3)H-TdR incorporation decreased significantly from 14,900+/-1035 cpm/well in the control group to 8,575+/-749 cpm/well in the confluent ECs group (n=6, P<0.01), and increased to 27,268+/-2310 cpm/well in the proliferative ECs group ( n=6, P<0.01). The transition of SMCs from G(0)/G(1) phase to G(2)/M and S phases was blocked in the confluent ECs group but promoted in the proliferative ECs group. Compared with the control group, the number of migrating cells was about 4 times higher in the proliferative ECs group (n=6, P<0.01), while it in the confluent ECs group was only the half of the number of the control(n=6, P<0.05). The expression of alpha-SM-actin mRNA was increased significantly in the confluent ECs group(2.3+/-0.11 vs 1.4+/-0.12, P<0.05), but decreased significantly in the proliferative ECs group(0.92+/-0.08 vs 1.4+/-0.12, P<0.05). The results suggest that the biologic features of SMCs are influenced by ECs growth states. The proliferative ECs promote SMCs proliferation, migration and downregulate alpha-SM-actin mRNA expression significantly.
内皮损伤、平滑肌细胞（SMC）增殖和迁移是许多心血管疾病（如动脉粥样硬化、高血压、糖尿病和再狭窄）共有的常见病理生理过程。确定病理条件下内皮细胞（EC）与SMC之间的功能相互作用很重要。这项工作旨在研究细胞共培养系统中EC生长状态对血管SMC增殖和迁移的影响。采用³H-TdR掺入法和流式细胞术确定EC生长状态对SMC增殖的影响。计数迁移的SMC数量。用RT-PCR分析α-SM-肌动蛋白mRNA的表达。结果显示，³H-TdR掺入量从对照组的14900±1035 cpm/孔显著降至汇合EC组的8575±749 cpm/孔（n = 6，P<0.01），并在增殖EC组中增至27268±2310 cpm/孔（n = 6，P<0.01）。在汇合EC组中，SMC从G(0)/G(1)期向G(2)/M期和S期的转变受到阻滞，但在增殖EC组中得到促进。与对照组相比，增殖EC组中迁移细胞的数量高出约4倍（n = 6，P<0.01），而汇合EC组中的迁移细胞数量仅为对照组的一半（n = 6，P<0.05）。汇合EC组中α-SM-肌动蛋白mRNA的表达显著增加（2.3±0.11对1.4±0.12，P<0.05），但在增殖EC组中显著降低（0.92±0.08对1.4±0.12，P<0.05）。结果表明，SMC的生物学特性受EC生长状态的影响。增殖的EC促进SMC增殖、迁移并显著下调α-SM-肌动蛋白mRNA的表达。
