Chi Eva Y, Krishnan Sampathkumar, Randolph Theodore W, Carpenter John F
Department of Chemical Engineering, Center for Pharmaceutical Biotechnology, ECCH 111, Campus Box 424, University of Colorado, Boulder, Colorado, USA.
Pharm Res. 2003 Sep;20(9):1325-36. doi: 10.1023/a:1025771421906.
Irreversible protein aggregation is problematic in the biotechnology industry, where aggregation is encountered throughout the lifetime of a therapeutic protein, including during refolding, purification, sterilization, shipping, and storage processes. The purpose of the current review is to provide a fundamental understanding of the mechanisms by which proteins aggregate and by which varying solution conditions, such as temperature, pH, salt type, salt concentration, cosolutes, preservatives, and surfactants, affect this process.
不可逆蛋白质聚集在生物技术行业中是个问题，在治疗性蛋白质的整个生命周期中都会遇到聚集现象，包括在重折叠、纯化、灭菌、运输和储存过程中。本综述的目的是对蛋白质聚集的机制以及不同的溶液条件（如温度、pH值、盐类型、盐浓度、共溶质、防腐剂和表面活性剂）如何影响这一过程提供基本的理解。
