Bando Hiroko, Toi Masakazu, Kitada Kunio, Koike Morio
Breast Cancer Research Group, Tokyo Metropolitan Cancer and Infectious Diseases Center, Honkomagome, 3-18-22, Bunkyo-ku, Tokyo 113-0087, Japan.
Biomed Pharmacother. 2003 Oct;57(8):333-40. doi: 10.1016/s0753-3322(03)00098-2.
Hypoxia is a stress that causes alterations in signal transduction and gene instability. In the cancer microenvironment, hypoxia plays a significant role in forming a tumor phenotype and tumor progression. We aimed to identify the genes upregulated by hypoxia in human breast cancer cell lines, a hormone-dependent MCF-7 and a hormone-independent MDA-MB-231, using microarray analysis. These cells were exposed to two oxygen concentrations such as 21% and 1% in a time-course. Out of 12625 genes, 26 genes were identified as commonly upregulated in both MCF-7 and MDA-MB-231 cells. Some of these genes were already reported as hypoxia-related, but some of those were identified newly. These commonly upregulated genes between hormone-dependent and hormone-independent cells would be a clue to study hypoxia-related events and to explore the novel therapeutic targets in human breast cancer.
缺氧是一种会导致信号转导改变和基因不稳定的应激。在癌症微环境中，缺氧在形成肿瘤表型和肿瘤进展中起着重要作用。我们旨在通过微阵列分析，确定在人乳腺癌细胞系（激素依赖性的MCF-7和激素非依赖性的MDA-MB-231）中因缺氧而上调的基因。这些细胞在一定时间内暴露于两种氧浓度，即21%和1%。在12625个基因中，有26个基因被确定在MCF-7和MDA-MB-231细胞中均上调。其中一些基因已被报道与缺氧相关，但也有一些是新发现的。激素依赖性和激素非依赖性细胞之间这些共同上调的基因将为研究缺氧相关事件以及探索人类乳腺癌新的治疗靶点提供线索。
