Feizi Ten, Fazio Fabio, Chai Wengang, Wong Chi Huey
The MRC Glycosciences Laboratory, Imperial College London, Northwick Park & St Mark's Hospital Campus, Watford Road, Harrow, Middlesex HA1 3UJ, UK.
Curr Opin Struct Biol. 2003 Oct;13(5):637-45. doi: 10.1016/j.sbi.2003.09.002.
Carbohydrate microarray technologies are new developments at the frontiers of glycomics. Results of 'proof of concept' experiments with carbohydrate-binding proteins of the immune system - antibodies, selectins, a cytokine and a chemokine - and several plant lectins indicate that microarrays of carbohydrates (glycoconjugates, oligosaccharides and monosaccharides) will greatly facilitate not only surveys of proteins for carbohydrate-binding activities but also elucidation of their ligands. It is predicted that both naturally occurring and synthetic carbohydrates will be required for the fabrication of microarrays that are sufficiently comprehensive and representative of entire glycomes. New leads to biological pathways that involve carbohydrate-protein interactions and new therapeutic targets are among biomedically important outcomes anticipated from applications of carbohydrate microarrays.
碳水化合物微阵列技术是糖组学前沿领域的新进展。使用免疫系统的碳水化合物结合蛋白（抗体、选择素、一种细胞因子和一种趋化因子）以及几种植物凝集素进行的“概念验证”实验结果表明，碳水化合物（糖缀合物、寡糖和单糖）微阵列不仅将极大地促进对具有碳水化合物结合活性的蛋白质的研究，还将有助于阐明其配体。预计天然存在的碳水化合物和合成碳水化合物都将用于制造足够全面且能代表整个糖组的微阵列。碳水化合物微阵列应用有望带来的生物医学重要成果包括涉及碳水化合物 - 蛋白质相互作用的新生物途径线索和新的治疗靶点。
