Remond M, Boireau P, Lebreton F
Centre National d'Etudes Vétérinaires et Alimentaires, Laboratoire Central de Recherches Vétérinaires, Maisons-Alfort, France.
Arch Virol. 1992;127(1-4):257-69. doi: 10.1007/BF01309589.
The cloning and sequencing of an Eco RI-PstI fragment derived from the replicative form of a canine parvovirus (CPV) vaccine strain are reported. The variability of the 5' end of NS 1 protein gene in the genome is confirmed by comparison with previously determined DNA sequences. A 15 nucleotide deletion was also observed in this vaccine strain. In order to improve CPV diagnosis, radioactively labelled RNA or DNA and biotin labelled DNA obtained by random priming of the recombinant plasmid were used as probes mainly on gut or stool samples from naturally infected dogs. Results of filter hybridization correlated well with histopathological diagnosis of parvovirus infection and with hemagglutination tests performed on dog faeces. We propose that nucleic acid hybridization may be an alternative diagnostic method to ascertain the presence of CPV, especially in frozen samples.
报道了从犬细小病毒（CPV）疫苗株复制型中获得的Eco RI - PstI片段的克隆和测序。通过与先前测定的DNA序列比较，证实了基因组中NS 1蛋白基因5'端的变异性。在该疫苗株中还观察到15个核苷酸的缺失。为了改进CPV诊断，通过随机引物法从重组质粒获得的放射性标记RNA或DNA以及生物素标记DNA主要用作来自自然感染犬的肠道或粪便样本的探针。滤膜杂交结果与细小病毒感染的组织病理学诊断以及对犬粪便进行的血凝试验相关性良好。我们提出核酸杂交可能是确定CPV存在的一种替代诊断方法，特别是在冷冻样本中。
