Azumi Kaoru, Takahashi Hiroki, Miki Yasufumi, Fujie Manabu, Usami Takeshi, Ishikawa Hisayoshi, Kitayama Atsusi, Satou Yutaka, Ueno Naoto, Satoh Nori
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Zoolog Sci. 2003 Oct;20(10):1223-9. doi: 10.2108/zsj.20.1223.
A cDNA microarray was constructed from a basal chordate, the ascidian Ciona intestinalis. The draft genome of Ciona has been read and inferred to contain approximately 16,000 protein-coding genes, and cDNAs for transcripts of 13,464 genes have been characterized and compiled as the "Ciona intestinalis Gene Collection Release I". In the present study, we constructed a cDNA microarray of these 13,464 Ciona genes. A preliminary experiment with Cy3- and Cy5-labeled probes showed extensive differential gene expression between fertilized eggs and larvae. In addition, there was a good correlation between results obtained by the present microarray analysis and those from previous EST analyses. This first microarray of a large collection of Ciona intestinalis cDNA clones should facilitate the analysis of global gene expression and gene networks during the embryogenesis of basal chordates.
利用一种基部脊索动物——海鞘（Ciona intestinalis）构建了一个cDNA微阵列。海鞘的基因组草图已完成测序，据推测其含有约16,000个蛋白质编码基因，并且已对13,464个基因转录本的cDNA进行了表征，并汇编成“海鞘基因集第一版”。在本研究中，我们构建了包含这13,464个海鞘基因的cDNA微阵列。用Cy3和Cy5标记的探针进行的初步实验表明，受精卵和幼虫之间存在广泛的差异基因表达。此外，本微阵列分析所得结果与先前EST分析的结果之间具有良好的相关性。这个由大量海鞘cDNA克隆组成的首个微阵列应有助于分析基部脊索动物胚胎发育过程中的全局基因表达和基因网络。
