Rutkowski Joseph M, Santiago Lizzie Y, Ben-Jebria Abdellaziz, Ultman James S
Department of Chemical Engineering, Pennsylvania State University, University Park, Pennsylvania, USA.
Inhal Toxicol. 2003 Nov;15(13):1369-85. doi: 10.1080/08958370390241876.
A method of determining the ozone-specific antioxidant capacity (OZAC) of lavage samples from the respiratory system was developed: Gaseous ozone (O(3)) was produced in cuvettes by irradiation with an ultraviolet lamp; aliquots of sample or of a saline control were then added and sufficient time was allowed for ozonation to reach completion; and an aliquot of indigo trisulfonate (ITS) was added to react with excess O(3). Because each molecule of O(3) rapidly bleaches one molecule of the deeply colored ITS, an OZAC value in concentration units was computed from the difference in light absorbance between the sample and the saline control multiplied by the extinction coefficient of ITS. Experiments in 0-40 micro M antioxidant solutions indicated that the OZAC values of uric acid and ascorbic acid were close to their actual concentrations and were independent of O(3) concentration. On the other hand, the OZAC of reduced glutathione and possibly human nasal lavage were nonlinearly related to antioxidant concentration and were directly related to O(3) concentration.
开发了一种测定呼吸系统灌洗样本中臭氧特异性抗氧化能力（OZAC）的方法：在比色皿中通过紫外线灯照射产生气态臭氧（O(3)）；然后加入样本或生理盐水对照的等分试样，并给予足够时间使臭氧化反应完成；接着加入靛蓝三磺酸盐（ITS）的等分试样以与过量的O(3)反应。由于每个O(3)分子会迅速使一个深色的ITS分子褪色，因此根据样本与生理盐水对照之间吸光度的差异乘以ITS的消光系数来计算浓度单位的OZAC值。在0 - 40微摩尔抗氧化剂溶液中进行的实验表明，尿酸和抗坏血酸的OZAC值接近其实际浓度，且与O(3)浓度无关。另一方面，还原型谷胱甘肽以及可能的人类鼻腔灌洗样本的OZAC与抗氧化剂浓度呈非线性关系，且与O(3)浓度直接相关。
