Sobol' M A
Tsitol Genet. 2003 Jul-Aug;37(4):40-5.
For the first time, in altered gravity, with antifibrillarin antibodies and immunogold microscopy fibrillarin that is one of the most important proteins of rRNA processing was localized. The quantitative study of the density of gold particles in the nuclelous, under clinorotation both the transition zone FC-DFC and the dense fibrillar component were less labeled as compared to the control. Obtained data allow us to suppose the lowering of the rRNA processing level in the nucleoli under the influence of altered gravity.
首次在模拟微重力条件下,通过抗原纤维蛋白抗体和免疫金显微镜技术对参与rRNA加工的最重要蛋白质之一——原纤维蛋白进行了定位。在clinorotation条件下,对核仁中金颗粒密度进行定量研究,结果显示与对照组相比,转录间隔区FC-DFC和致密纤维组分的标记较少。所得数据使我们推测,在模拟微重力影响下,核仁中rRNA加工水平降低。