Puvion-Dutilleul F, Christensen M E
Laboratoire de Biologie et Ultrastructure du Noyau de l'UPR 272, CNRS, Villejuif/France.
Eur J Cell Biol. 1993 Jun;61(1):168-76.
Nucleolar modifications induced by adenovirus type 5 (Ad5) have been examined in HeLa cells by bismuth staining and immunogold labeling of fibrillarin, both of which visualize the dense fibrillar component (DFC) of nucleoli. A progressive increase in the compaction of the nucleolus is accompanied both by a migration of strands of DFC towards the border of the nucleolar body and by a decrease in the amount of fibrillarin within the bismuth stained DFC. In addition, the bismuth-stained extranucleolar fibrillar spots induced by Ad5 infection often were intensely labeled with monoclonal anti-fibrillarin antibody. Previously reported defects in ribosomal RNA expression during adenovirus infection could result from the migration and accumulation of nucleolar proteins implicated in ribosomal biogenesis into virus-induced extranucleolar structures.
通过铋染色和对纤维蛋白原进行免疫金标记，在HeLa细胞中检测了5型腺病毒（Ad5）诱导的核仁修饰，这两种方法均可使核仁的致密纤维成分（DFC）可视化。核仁压缩的逐渐增加伴随着DFC链向核仁体边界的迁移以及铋染色的DFC内纤维蛋白原数量的减少。此外，Ad5感染诱导的铋染色核仁外纤维斑点通常被单克隆抗纤维蛋白原抗体强烈标记。先前报道的腺病毒感染期间核糖体RNA表达缺陷可能是由于参与核糖体生物合成的核仁蛋白迁移并积累到病毒诱导的核仁外结构中所致。
